Glyphosate is one of the most widely used pesticides, which can cause toxicity to aquatic animals. In this study, the survival rate, immune response, digestive enzyme activities, and the intestinal microbiota diversity of Chinese mitten crab (Eriocheir sinensis) were evaluated after 14 days of exposure to glyphosate (48.945 mg/L from 50% 96 h LC₅₀ value) and melatonin feeding (80 mg/kg). The results showed that MT significantly improved the survival rate, antibacterial capacity of E. sinensis (P < 0.05). After exposure to glyphosate, the expression of Hsp60, Hsp70 and Hsp90 in cranial ganglia and thoracic ganglia was decreased significantly, but MT significantly raised the expression of these proteins (P < 0.05). Glyphosate significantly decreased lipase activity compared with the control group (P < 0.05), while melatonin significantly increased the lipase, amylase and trypsin activities (P < 0.05). Melatonin significantly increased the Chao1 and Shannon index and the relative abundance of Proteobacteria and Bacteroidetes (P < 0.05). This study shows that melatonin has a protective effect on the glyphosate exposed E. sinensis.

草甘膦是使用最广泛的农药之一，会对水生动物造成毒性。本研究评估了中华绒螯蟹（ Eriocheir sinensis ）接触草甘膦（48.945 mg/L，50% 96 h LC ₅₀值）14天后的存活率、免疫反应、消化酶活性和肠道微生物多样性。 ）和褪黑激素喂养（80毫克/公斤）。结果表明，MT显着提高了大肠杆菌的存活率、抗菌能力。中华( P < 0 . 05 )。草甘膦暴露后，颅神经节和胸神经节中Hsp60、Hsp70和Hsp90的表达显着降低，而MT显着上调这些蛋白的表达( P < 0 . 05 )。与对照组相比，草甘膦显着降低脂肪酶活性( P < 0. 05 )，而褪黑素则显着提高脂肪酶、淀粉酶和胰蛋白酶活性( P < 0. 05 )。褪黑激素显着​​增加了 Chao1 和 Shannon 指数以及变形菌门和拟杆菌门的相对丰度 ( P < 0 . 05 )。这项研究表明，褪黑激素对接触草甘膦的大肠杆菌具有保护作用。中华。