Dichloromethane fraction (DF) of Piper nigrum L. and P. longum L. (PnL and PlL), has been found to exert a protective effect against ischemic stroke in rats. However, the regulatory mechanism exerted by PnL and PIL have not been fully elucidated. In this study, we found that DF greatly ameliorated cerebral ischemic injury in a rat model of permanent middle cerebral artery occlusion (pMCAO). The neurological score, behavioral assessment, brain infarct volume, phosphorylation of AKT (p-AKT), phosphorylation mTOR (p-mTOR), and Atg7 protein levels were determined. Additionally, we discovered that DF pretreatment reduced infarct volume, neurological score, and brain damage. Furthermore, DF therapy caused the downregulation of Atg7 and p-AKT expression, as well as the upregulation of p-mTOR expression. In conclusion, our findings indicated that DF treatment can reduce brain damage and inhibit apoptosis and autophagy by activating the Akt-mTOR signaling pathway in ischemic stroke.

Piper nigrum L.和P. longum L. 的二氯甲烷馏分 (DF) 。(PnL 和 PlL) 已被发现对大鼠的缺血性中风发挥保护作用。然而，PnL 和 PIL 发挥的调节机制尚未完全阐明。在这项研究中，我们发现 DF 极大地改善了永久性大脑中动脉闭塞 (pMCAO) 大鼠模型的脑缺血损伤。确定了神经学评分、行为评估、脑梗塞体积、AKT 磷酸化 (p-AKT)、磷酸化 mTOR (p-mTOR) 和 Atg7 蛋白水平。此外，我们发现 DF 预处理减少了梗塞体积、神经系统评分和脑损伤。此外，DF 治疗导致 Atg7 和 p-AKT 表达下调，以及 p-mTOR 表达上调。综上所述，