Acta Biomaterialia ( IF 9.4 ) Pub Date : 2020-08-07 , DOI: 10.1016/j.actbio.2020.07.062 Jianglong Yan 1 , Dandan Xia 2 , Wenhao Zhou 3 , Yangyang Li 1 , Pan Xiong 4 , Qiyao Li 5 , Pei Wang 1 , Ming Li 6 , Yufeng Zheng 2 , Yan Cheng 1
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Polyetheretherketone has been widely used for bone defect repair, whereas failures may happen due to implant loosening and infection. Thus, PEEK implant with multi-function (osteogenesis, angiogenesis, and bacteria-killing) is essential to solve this problem. Herein, copper oxide microspheres (µCuO) decorated with silver nanoparticles (nAg) were constructed on porous PEEK surface via silk fibroin. In vitro studies highlighted the pH controlled release ability of this coating. It liberated a high dose of Cu2+ and Ag+ at low pH environment (pH 5.0), leading to 99.99% killing of planktonic bacteria and complete eradication of sessile bacteria, avoiding biofilm formation. Under physiological environment (pH 7.4), a lower amount of leaked metal ions induced promoted ALP production, collagen secretion, and calcium deposition, as well as NO production, which indicated potentiated osteogenesis and angiogenesis. In vivo results displayed the highest new bone volume around, and the appearance of new bone inside porous structure of, PEEK implant with this coating in rabbit tibia, signified the abilities of this coating to promote bone regeneration and osseointegration. Our study established solid support for implants with this coating to be a successful bone defect repair solution.
Statement of Significance
In this study, CuO/Ag micro/nano particles were incorporated into the porous surface of PEEK through polydopamine and silk fibroin layers. The design of this coating conferred pH-controlled release behavior to Cu2+ and Ag+. High dose of metal ions were released at pH 5.0, which presented synergistic antibacterial ability and killed 99.99% of planktonic bacteria. Low concentration of metal ions were controlled by this coating at physiological environment, which potentiated osteodifferentiation of Ad-MSC in vitro and led to complete integration of implant with bone tissue in vivo.
中文翻译：
pH响应的丝素蛋白基CuO / Ag微/纳米涂层赋予聚醚醚酮以协同抗菌能力，成骨作用和血管生成作用。
聚醚醚酮已被广泛用于骨缺损的修复，但由于植入物松动和感染，可能会发生失败。因此，具有多功能（成骨，血管生成和细菌杀死）功能的PEEK植入物对于解决此问题至关重要。本文中，通过丝素蛋白在多孔PEEK表面上构建了用银纳米颗粒（nAg）装饰的氧化铜微球（µCuO）。体外研究强调了该涂层的pH控制释放能力。它释放了高剂量的Cu 2+和Ag +在低pH值环境（pH 5.0）下，可导致99.99％的浮游细菌杀死并完全根除无柄细菌，从而避免形成生物膜。在生理环境（pH 7.4）下，诱导的少量金属离子泄漏促进了ALP的产生，胶原蛋白的分泌，钙的沉积以及NO的产生，这表明增强了成骨和血管生成。体内结果显示，在兔胫骨中带有该涂层的PEEK植入物周围具有最高的新骨量，并且在多孔结构中出现了新的骨骼，表明该涂层具有促进骨再生和骨整合的能力。我们的研究建立了具有这种涂层的植入物的固体支持物，是成功的骨缺损修复解决方案。
重要声明
在这项研究中，CuO / Ag微/纳米颗粒通过聚多巴胺和丝素蛋白层掺入PEEK的多孔表面。该涂层的设计赋予了Cu 2+和Ag + pH控制释放行为。在pH 5.0下释放高剂量的金属离子，具有协同抗菌作用，杀死了99.99％的浮游细菌。在生理环境下，通过这种涂层控制了低浓度的金属离子，这在体外增强了Ad-MSC的骨分化，并导致体内植入物与骨组织的完全整合。
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