Crataeva nurvala Buch-Ham. (Capparidaceae) is an important medicinal plant, the bark of which is extensively used in Ayurveda as a remedy for kidney stones, urinary tract infections, prostate-related disorders, and benign prostatic hypertrophy. In this study, a sensitive and reliable high-performance thin-layer chromatography (HPTLC) method has been developed for the simultaneous quantification of two bioactive markers, lupeol and β-sitosterol, in the stem bark of C. nurvala using precoated silica gel G 60 F₂₅₄ HPTLC plates as the stationary phase and toluene–methanol (9.6:0.4, v/v) as the mobile phase. Detection and quantification were carried out by densitometric scanning at 545 nm after derivatization using 1% anisaldehyde–sulfuric acid reagent. The method was validated for linearity, inter-day and intra-day precision, repeatability, accuracy, specificity, limit of detection, and limit of quantification. The linear regression analysis data for the calibration plots showed a good linear relationship (r² = 0.987–0.990) in the concentration range of 2 to 6 μg for lupeol and β-sitosterol, both with respect to areas. The proposed method provides good resolution of lupeol and β-sitosterol from other constituents present in the stem bark of C. nurvala. It was found to be simple, accurate, precise, and reproducible and would potentially be a useful tool for routine analysis of C. nurvala.

Crataeva nurvala布赫汉姆。（Capparidaceae）是一种重要的药用植物，其树皮被广泛用于印度草医学中，作为肾结石，尿路感染，前列腺相关疾病和良性前列腺肥大的药物。在这项研究中，已经开发了一种灵敏可靠的高效薄层色谱法（HPTLC），用于使用预涂硅胶G对N.valva茎皮中的两种生物活性标记物lupeol和β-谷甾醇进行同时定量。60 F ₂₅₄ HPTLC板作为固定相，甲苯-甲醇（9.6：0.4，v / v）作为流动相。在衍生化后，使用1％茴香醛-硫酸试剂通过光密度扫描在545 nm进行检测和定量。验证了该方法的线性，日间和日间精度，重复性，准确性，特异性，检测限和定量限。校正图的线性回归分析数据显示 ，在2至6μg的浓度范围内，卢比欧酚和β-谷甾醇的线性关系良好（r ² = 0.987–0.990），均相对于面积。所提出的方法提供了卢泊酚和β-谷甾醇与否的组成，其存在于否。它被发现是简单，准确，精确和可重现的，可能会成为C常规分析的有用工具。nurvala。