Quorum sensing (QS) inhibition is an essential strategy to combat bacterial infection. Previously, we have synthesized a series of thymidine derivatives bearing isoxazole and 1,2,3-triazole rings (TITL). Herein, the inhibitory effects of TITL on QS of Pseudomonas aeruginosa PAO1 were evaluated. In vitro results demonstrated that TITL effectively inhibited biofilm formation and reduced the virulence factors of P. aeruginosa PAO1. In combination with antibiotics, our TITL compounds significantly prolonged the lifespans of Caenorhabditis elegans N2 nematodes that were infected with P. aeruginosa PAO1 in vivo. In conclusion, TITL compounds are promising candidates for the treatment of antibiotic-resistant P. aeruginosa PAO1.

抑制群体感应（QS）是对抗细菌感染的重要策略。以前，我们已经合成了一系列带有异恶唑和1,2,3-三唑环（TITL）的胸苷衍生物。在此，评价了TITL对铜绿假单胞菌PAO1的QS的抑制作用。体外结果表明，TITL有效抑制了生物膜的形成并降低了铜绿假单胞菌PAO1的毒力因子。与抗生素组合，我们的TITL化合物可显着延长体内感染铜绿假单胞菌PAO1的秀丽隐杆线虫N2线虫的寿命。总之，TITL化合物有望用于治疗耐药性铜绿假单胞菌 PAO1。