This paper focuses on some factors that affect the gut bacterial communities of Plodia interpunctella and their role in the removal of plastic polymer concentrations to make a clean environment. Counting of midgut bacteria from P. interpunctella reared on different diets exhibited that wheat bran gave a significantly higher number of colonies than other diets (84 × 102 CFU/gut). Histological midgut studies on control fully grown larvae showed different shapes of epithelial cells, elongated or pyramidal, and few numbers of oval regenerative cells. Various secretions are released from the apical margin of the cells. The peritrophic membrane cannot be detected and large numbers of bacteria occupied the midgut lumen. While, irradiated larvae with 250 Gy revealed sever damaged midgut cells, some cells appeared swollen and the others fused as heterogeneous mass. Besides, ruptured of peritrophic membrane and very reduction in the bacterial population were appeared in the lumen. In this respect, two bacterial isolates were isolated from the midgut larvae and identified by 16S rRNA gene sequencing with accession number NR 146667.2 and NR 112686.1, as Enterobacter tabaci strain YIM Hb-3(B1) and Bacillus subtilis subsp. Spizizenii strain NBRC 101239(B2), respectively. Scanning electron microscopy analysis revealed alterations in the polyethylene during 60 days of incubation. Two bacterial strains showed damaging effects on plastic including cavities and pits on the surface of plastic pieces increased with the time of treatment. Moreover, the formation of carbonyl groups was verified using a fourier transform infrared imaging microscope.

中文翻译：

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伽马辐射对 Plodia interpunctella 中肠细菌的影响及其在有机废物生物降解中的作用

本文重点介绍影响 Plodia interpunctella 肠道细菌群落的一些因素及其在去除塑料聚合物浓度以创造清洁环境中的作用。对不同日粮饲养的 P. interpunctella 中肠细菌的计数表明，麦麸的菌落数量明显高于其他日粮（84 × 102 CFU/肠）。对照完全生长的幼虫的组织学中肠研究显示不同形状的上皮细胞，细长或锥体，以及少量的椭圆形再生细胞。各种分泌物从细胞的顶端边缘释放。无法检测到围营养膜，大量细菌占据中肠腔。同时，用 250 Gy 辐照的幼虫显示出严重受损的中肠细胞，一些细胞出现肿胀，而另一些则融合为异质团块。此外，管腔内出现周营养膜破裂和细菌数量大幅减少。在这方面，从中肠幼虫中分离出两种细菌分离株，并通过 16S rRNA 基因测序鉴定为登记号为 NR 146667.2 和 NR 112686.1 的烟粉虱肠杆菌菌株 YIM Hb-3(B1) 和枯草芽孢杆菌亚种。Spizizenii 菌株分别为 NBRC 101239(B2)。扫描电子显微镜分析显示，在 60 天的孵化过程中，聚乙烯发生了变化。两种细菌菌株对塑料显示出破坏作用，包括塑料件表面的空洞和凹坑，随着处理时间的增加而增加。此外，使用傅立叶变换红外成像显微镜验证了羰基的形成。