Grafting success by cell wall remodeling Plants that produce great fruit may not always have great roots. Grafting of a productive scion onto a resilient rootstock has provided agriculturalists with solutions to this and other challenges. Notaguchi et al. have now studied why some plant grafts work better than others (see the Perspective by McCann). The tobacco relative Nicotiana benthamiana (Nb) turns out to be the superhero of grafting, able to form grafts with plants from a wide range of evolutionary families. A bit of Nb, set as a middleman between a tomato scion and an Arabidopsis rootstock, negotiated a successful junction between these two otherwise nonconversant plant species. The expression of β-1,4-glucanases secreted into the extracellular region turns out to be key in facilitating cell wall reconstruction. Science, this issue p. 698; see also p. 618 Plant graft junctions are aided by cell wall remodeling. Plant grafting is conducted for fruit and vegetable propagation, whereby a piece of living tissue is attached to another through cell-cell adhesion. However, graft compatibility limits combinations to closely related species, and the mechanism is poorly understood. We found that Nicotiana is capable of graft adhesion with a diverse range of angiosperms. Comparative transcriptomic analyses on graft combinations indicated that a subclade of β-1,4-glucanases secreted into the extracellular region facilitates cell wall reconstruction near the graft interface. Grafting was promoted by overexpression of the β-1,4-glucanase. Using Nicotiana stem as an interscion, we produced tomato fruits on rootstocks from other plant families. These findings demonstrate that the process of cell-cell adhesion is a potential target to enhance plant grafting techniques.

中文翻译：

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β-1,4-葡聚糖酶促进植物嫁接中的细胞间粘附


通过细胞壁重塑实现嫁接成功 结出丰硕果实的植物并不总是拥有伟大的根系。将高产接穗嫁接到有弹性的砧木上为农民提供了应对这一挑战和其他挑战的解决方案。野田口等人。现在已经研究了为什么某些植物嫁接比其他植物嫁接效果更好（参见麦肯的观点）。烟草的近亲本塞姆氏烟草（Nicotiana Benthamiana，Nb）被证明是嫁接的超级英雄，能够与来自广泛进化家族的植物形成嫁接物。一点铌作为番茄接穗和拟南芥砧木之间的中间人，在这两种原本不熟悉的植物物种之间成功建立了连接。分泌到细胞外区域的 β-1,4-葡聚糖酶的表达被证明是促进细胞壁重建的关键。科学，本期第 14 页。 698;另见 p. 618 细胞壁重塑有助于植物嫁接连接。植物嫁接用于水果和蔬菜的繁殖，通过细胞与细胞的粘附将一块活组织附着到另一块活组织上。然而，接枝相容性限制了密切相关物种的组合，并且其机制尚不清楚。我们发现烟草能够与多种被子植物进行移植粘附。对移植物组合的比较转录组分析表明，分泌到细胞外区域的β-1,4-葡聚糖酶的一个亚支促进了移植物界面附近的细胞壁重建。 β-1,4-葡聚糖酶的过度表达促进了嫁接。使用烟草茎作为间插穗，我们在其他植物科的砧木上生产了番茄果实。这些发现表明细胞间粘附过程是增强植物嫁接技术的潜在目标。