Functional Constitutional Dynamic Networks Revealing Evolutionary Reproduction/Variation/Selection Principles,Journal of the American Chemical Society

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Functional Constitutional Dynamic Networks Revealing Evolutionary Reproduction/Variation/Selection Principles
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2020-08-05 , DOI: 10.1021/jacs.0c05669
Liang Yue ₁ , Shan Wang ₁ , Itamar Willner ₁

Affiliation

Within the broad research efforts to engineer chemical pathways to yield high-throughput evolutionary synthesis of genes and their screening for dictated functionalities, we introduce the evolution of nucleic-acid-based constitutional dynamic networks (CDNs) that follow reproduction/variation/selection principles. These fundamental principles are demonstrated by assembling a library of nucleic-acid strands and hairpins as functional modules for evolving networks. Primary T1-initiated selection of components from the library assembles a parent CDN X, where the evolved constituents exhibit catalytic properties to cleave the hairpins in the library. Cleavage of the hairpins yields fragments, which reproduces T1 to replicate CDN X, whereas the other fragments T2 and T3 select other components to evolve two other CDNs, Y and Z (variation). By applying appropriate counter triggers, we demonstrate the guided selection of networks from the evolved CDNs. By integrating additional hairpin substrates into the system, CDN-dictated emergent catalytic transformations are accomplished. The study provides pathways to construct evolutionary dynamic networks revealing enhanced gated and cascaded functions.

中文翻译：

功能性宪法动态网络揭示进化繁殖/变异/选择原理

在设计化学途径以产生基因的高通量进化合成及其对指定功能的筛选的广泛研究工作中，我们介绍了遵循复制/变异/选择原则的基于核酸的组成动态网络（CDN）的进化。这些基本原理通过组装核酸链和发夹库作为进化网络的功能模块来证明。初级 T1 启动的从文库中选择的组件组装了亲本 CDN X，其中进化的成分表现出切割文库中发夹的催化特性。发夹的裂解产生片段，其复制 T1 来复制 CDN X，而其他片段 T2 和 T3 选择其他组件来进化另外两个 CDN，Y 和 Z（变异）。通过应用适当的计数器触发器，我们演示了从演进的 CDN 中引导选择网络。通过将额外的发夹底物集成到系统中，可以完成 CDN 指示的紧急催化转化。该研究提供了构建进化动态网络的途径，揭示了增强的门控和级联功能。

更新日期：2020-08-05

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