Single-cell RNA sequencing allows highly detailed profiling of cellular immune responses from limited-volume samples, advancing prospects of a new era of systems immunology. The power of single-cell RNA sequencing offers various opportunities to decipher the immune response to infectious diseases and vaccines. Here, we describe the potential uses of single-cell RNA sequencing methods in prophylactic vaccine development, concentrating on infectious diseases including COVID-19. Using examples from several diseases, we review how single-cell RNA sequencing has been used to evaluate the immunological response to different vaccine platforms and regimens. By highlighting published and unpublished single-cell RNA sequencing studies relevant to vaccinology, we discuss some general considerations how the field could be enriched with the widespread adoption of this technology.

中文翻译：

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单细胞RNA测序在疫苗学中的应用。

单细胞RNA测序技术可以对来自有限量样品的细胞免疫应答进行高度详细的分析，从而为系统免疫学的新时代提供了前景。单细胞RNA测序的功能提供了多种机会来解密对传染病和疫苗的免疫反应。在这里，我们描述了预防性疫苗开发中单细胞RNA测序方法的潜在用途，重点在于包括COVID-19在内的传染病。通过使用几种疾病的实例，我们回顾了如何使用单细胞RNA测序来评估对不同疫苗平台和方案的免疫应答。通过突出显示与疫苗学相关的已发表和未发表的单细胞RNA测序研究，