NAC (NAM, AFAT, and CUC) proteins play necessary roles in plant response to environmental stresses. However, the functional roles of NAC genes in moso bamboo (Phyllostachys edulis), an essential economic perennial woody bamboo species, are not well documented. In this study, we retrieved 152 PeNAC genes from the moso bamboo V2 genome, and PeSNAC-1 was isolated and functionally characterized. PeSNAC-1 was localized in the nucleus and had no transactivation activity in yeast. PeSNAC-1 extremely expressed in rhizome and young roots (0.1 and 0.5 cm) and was significantly induced by drought and salt treatments but repressed by abscisic acid (ABA), methyl jasmonate (MeJA) and high temperature (42°C) in moso bamboo. Under water shortage and salinity conditions, survival ratios, Fv/Fm values, physiological indexes such as activities of superoxide dismutase, peroxidase, and catalase and contents of malondialdehyde, H₂O₂, and proline were significantly higher in transgenic rice than the wild type, which suggests enhanced tolerance to drought and salt stress in PeSANC-1 overexpressed plants. Transcript levels of Na⁺/H⁺ antiporter and Na⁺ transporter genes (OsSOS1, OsNHX1 and OsHKT1;5), ABA signaling and biosynthesis genes (OsABI2, OsRAB16, OsPP2C68, OsLEA3–1, OsLEA3, OsNCED3, OsNCED4 and OsNCED5) and ABA independent genes (OsDREB1A, OsDREB1B and OsDREB2A) were substantially higher in transgenic as compared to the wild type. Moreover, protein interaction analysis revealed that PeSNAC-1 could interact with stress responsive PeSNAC-2/4 and PeNAP-1/4/5 in both yeast and plant cells, which indicates a synergistic effect of those proteins in regulating the moso bamboo stress response. Our data demonstrate that PeSNAC-1 likely improved salt and drought stress tolerance via modulating gene regulation in both ABA dependent and independent signaling pathways in transgenic rice. In addition, PeSNAC-1 functions as an important positive stress regulator in moso bamboo, participating in PeSNAC-1 and PeSNAC-2/−4 or PeSNAC-1 and PeNAP-1/4/5 interaction networks.

中文翻译：

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PeSNAC-1（一种来自毛竹（Phyllostachys edulis）的NAC转录因子）赋予转基因水稻耐盐和干旱的能力。

NAC（NAM，AFAT和CUC）蛋白在植物对环境胁迫的响应中起着必要的作用。然而，NAC基因在毛竹（Phyllostachys edulis）（一种重要的多年生木本经济竹种）中的功能性作用尚未得到充分记载。在这项研究中，我们从毛竹V2基因组中检索到152个PeNAC基因，并分离了PeSNAC-1并对其功能进行了表征。PeSNAC-1位于核中，在酵母中没有反式激活活性。PeSNAC-1在根茎和幼根（0.1和0.5厘米）中极度表达，并在干旱和盐处理下显着诱导，但在毛竹中被脱落酸（ABA），茉莉酸甲酯（MeJA）和高温（42°C）抑制。在缺水和盐碱条件下，转基因水稻的存活率，Fv / Fm值，超氧化物歧化酶，过氧化物酶和过氧化氢酶等生理指标以及丙二醛，H ₂ O ₂和脯氨酸的含量均显着高于野生型。 ，这表明PeSANC-1过表达植物对干旱和盐胁迫的耐受性增强。Na ⁺ / H ⁺反转运蛋白和Na ^+的转录水平转运蛋白基因（OsSOS1，OsNHX1和OsHKT1; 5），ABA信令和生物合成基因（OsABI2，OsRAB16，OsPP2C68，OsLEA3-1，OsLEA3，OsNCED3，OsNCED4和OsNCED5）和ABA独立基因（OsDREB1A，OsDREB1B和OsDREB2A与野生型相比，转基因）的高得多。此外，蛋白质相互作用分析表明，PeSNAC-1可以与酵母和植物细胞中的胁迫响应性PeSNAC-2 / 4和PeNAP-1 / 4/5相互作用，这表明这些蛋白在调节毛竹胁迫响应中具有协同作用。 。我们的数据表明，PeSNAC-1可能通过调节转基因水稻中ABA依赖和独立信号传导途径中的基因调控来改善盐和干旱胁迫耐受性。另外，PeSNAC-1在毛竹中起重要的正应力调节剂的作用，参与PeSNAC-1和PeSNAC-2 / -4或PeSNAC-1和PeNAP-1 / 4/5相互作用网络。