The present study is an attempt to determine the lipid composition of Candida auris and to highlight if the changes in lipids can be correlated to high drug resistance encountered in C. auris. For this, the comparative lipidomics landscape between drug-susceptible (CBS10913T) and a resistant hospital isolate (NCCPF_470033) of C. auris was determined by employing high throughput mass spectrometry. All major groups of phosphoglycerides (PGL), sphingolipids, sterols, diacylglycerols (DAG) and triacylglycerols (TAG), were quantitated along with their molecular lipid species. Our analyses highlighted several key changes where the NCCPF_470033 showed an increase in PGL content, specifically phosphatidylcholine, phosphatidylglycerol, phosphatidylserine, phosphatidylinositol, and phosphatidylethanolamine; odd chain containing lipids and accumulation of 16:1-DAG and 16:0-DAG; depletion of 18:1-TAG and 18:0-TAG. The landscape of molecular species displayed a distinct imprint between isolates. For example, the levels of unsaturated PGLs, contributed by both odd and even-chain fatty acyls were higher in resistant NCCPF_470033 isolate, resulting in a higher unsaturation index. Notwithstanding, several commonalities of lipid compositional changes between resistant C. auris and other Candida spp., the study could also identify distinguishable changes in specific lipid species in C. auris. Together, the data highlights the modulation of membrane lipid homeostasis associated with drug-resistant phenotype of C. auris.

中文翻译：

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人类致病真菌耳念珠菌的详细脂质组学研究。

本研究试图确定耳念珠菌的脂质组成，并强调脂质的变化是否与耳念珠菌遇到的高耐药性相关。为此，通过采用高通量质谱法确定了耳念珠菌的药物敏感性 (CBS10913T) 和耐药医院分离株 (NCCPF_470033) 之间的比较脂质组学景观。所有主要的磷酸甘油酯 (PGL)、鞘脂、甾醇、二酰基甘油 (DAG) 和三酰基甘油 (TAG) 及其分子脂质种类均进行了定量。我们的分析强调了几个关键变化，其中 NCCPF_470033 显示 PGL 含量增加，特别是磷脂酰胆碱、磷脂酰甘油、磷脂酰丝氨酸、磷脂酰肌醇和磷脂酰乙醇胺；含有脂质的奇数链以及16:1-DAG和16:0-DAG的积累；18:1-TAG 和 18:0-TAG 耗尽。分子物种的景观在分离物之间显示出独特的印记。例如，在抗性 NCCPF_470033 分离株中，由奇数链和偶数链脂肪酰基贡献的不饱和 PGL 水平较高，从而导致较高的不饱和指数。尽管抗性耳念珠菌和其他念珠菌属之间的脂质成分变化存在一些共性，但该研究还可以识别耳念珠菌中特定脂质种类的显着变化。总之，这些数据强调了与耳念珠菌耐药表型相关的膜脂稳态调节。