Dielectrophoretic Manipulation of Cancer Cells and Their Electrical Characterization.,ACS Combinatorial Science

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Dielectrophoretic Manipulation of Cancer Cells and Their Electrical Characterization.
ACS Combinatorial Science Pub Date : 2020-08-06 , DOI: 10.1021/acscombsci.0c00109
Ina Turcan ₁ , Marius Andrei Olariu ₁

Affiliation

Electromanipulation and electrical characterization of cancerous cells is becoming a topic of high interest as the results reported to date demonstrate a good differentiation among various types of cells from an electrical viewpoint. Dielectrophoresis and broadband dielectric spectroscopy are complementary tools for sorting, identification, and characterization of malignant cells and were successfully used on both primary tumor cells and culture cells as well. However, the literature is presenting a plethora of studies with respect to electrical evaluation of these type of cells, and this review is reporting a collection of information regarding the functioning principles of different types of dielectrophoresis setups, theory of cancer cell polarization, and electrical investigation (including here the polarization mechanisms). The interpretation of electrical characteristics against frequency is discussed with respect to interfacial/Maxwell–Wagner polarization and the parasitic influence of electrode polarization. Moreover, the electrical equivalent circuits specific to biological cells polarizations are discussed for a good understanding of the cells’ morphology influence. The review also focuses on advantages of specific low-conductivity buffers employed currently for improving the efficiency of dielectrophoresis and provides a set of synthesized data from the literature highlighting clear differentiation between the crossover frequencies of different cancerous cells.

中文翻译：

癌细胞的介电泳操作及其电学表征。

癌细胞的电操作和电学表征正成为一个备受关注的话题，因为迄今为止报道的结果表明，从电学角度来看，各种类型的细胞之间存在良好的分化。介电电泳和宽带介电光谱是对恶性细胞进行分类、鉴定和表征的互补工具，并且也成功用于原发肿瘤细胞和培养细胞。然而，文献提出了大量关于这些类型细胞的电学评估的研究，本综述报告了关于不同类型介电泳装置的功能原理、癌细胞极化理论和电学研究的信息集合。（包括这里的极化机制）。就界面/麦克斯韦-瓦格纳极化和电极极化的寄生影响讨论了对频率的电特性的解释。此外，还讨论了特定于生物细胞极化的等效电路，以便更好地了解细胞的形态影响。该评论还重点介绍了目前用于提高介电泳效率的特定低电导率缓冲液的优势，并提供了一组来自文献的合成数据，突出了不同癌细胞交叉频率之间的明显区别。讨论了特定于生物细胞极化的等效电路，以便更好地了解细胞的形态影响。该评论还重点介绍了目前用于提高介电泳效率的特定低电导率缓冲液的优势，并提供了一组来自文献的合成数据，突出了不同癌细胞交叉频率之间的明显区别。讨论了特定于生物细胞极化的等效电路，以便更好地了解细胞的形态影响。该评论还重点介绍了目前用于提高介电泳效率的特定低电导率缓冲液的优势，并提供了一组来自文献的合成数据，突出了不同癌细胞交叉频率之间的明显区别。

更新日期：2020-08-06

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