Abstract

Herein the green synthesis of iron oxide (FeO) nanoparticles (NPs) was achieved by using Thymbra spicata leaf extract. The antibacterial activity of FeONPs was evaluated against both gram-positive and gram-negative bacteria by using broth microdilution assay. Further, the antioxidant and cytotoxic potential of FeONPs was determined by using standard protocols. The effects of nanoparticles on Staphylococcus aureus growth and on their biofilm formation were investigated. FeONPs at the concentration of 100 µg/mL exhibited excellent bactericidal action against Salmonella typhimurium. Also, the biogenic nanoparticles inhibited the growth rate of S. aureus. Furthermore, FeONPs showed noncytotoxic characteristics on L929 mouse fibroblast cells. All these results revealed that FeONPs obtained by simple and eco-friendly synthesis process through T. spicata extract could be considered a promising candidate as antibacterial agent for many different applications. Also, biogenic FeONPs with their antibiofilm activity on S. aureus could serve as potent antibacterial agents against pathogenic biofilm-forming bacteria.

抽象的

在这里，绿色合成氧化铁（FeO）纳米颗粒（NPs）是通过使用百里香叶提取物实现的。FeONPs的抗菌活性通过肉汤微量稀释法对革兰氏阳性菌和革兰氏阴性菌进行了评估。此外，通过使用标准方案确定了FeONP的抗氧化剂和细胞毒性潜力。研究了纳米颗粒对金黄色葡萄球菌生长及其生物膜形成的影响。FeONPs的浓度为100 µg / mL，对鼠伤寒沙门氏菌表现出优异的杀菌作用。而且，生物纳米颗粒抑制金黄色葡萄球菌的生长速率。此外，FeONP在L929小鼠成纤维细胞上显示出非细胞毒性特征。所有这些结果表明通过简单和环保的方法合成，获得该FeONPs T.兰香提取物可以被认为是有希望的候选人为许多不同的应用抗菌剂。同样，对金黄色葡萄球菌具有抗生物膜活性的生物FeONP可以作为有效的抗病原生物膜形成细菌的抗菌剂。