Abstract

A facile and reliable colorimetric method for detection of calcium ion and calmodulin was developed, which is based on the calcium ion-induced aggregation of gold nanoparticles, accompanied by an obvious color change from red to blue-purple. Calcium ion can be detected in the concentration range from 2.5 to 37.5 µmol·L⁻¹ with a 0.6 µmol·L⁻¹ detection limit. Calmodulin can preferentially capture calcium ion to inhibit the aggregation and the color changes of gold nanoparticles, and can be determined with a 0.8 µg·mL⁻¹ detection limit over the concentration range from 0 to 33.6 µg·mL⁻¹.

中文翻译：

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基于金纳米粒子的钙离子和钙调蛋白检测的比色法

抽象的

建立了一种简便，可靠的比色法检测钙离子和钙调蛋白，该方法基于钙离子诱导的金纳米粒子聚集，并伴有从红色到蓝色-紫色的明显颜色变化。钙离子的检测浓度范围为2.5至37.5 µmol·L ^-1，检测极限为0.6 µmol·L ^-1。钙调蛋白可以优先捕获钙离子以抑制金纳米颗粒的聚集和颜色变化，并且可以在0.8至33.6 µg·mL ^-1的浓度范围内以0.8 µg·mL ^-1的检测限进行测定。