PURPOSE This paper aims to assess the role of CEMIP in human breast cancer, and explore the potential mechanisms of CEMIP in the progression of breast cancer. METHODS We examined the expression levels of CEMIP in breast cancer tissues and cell lines by quantitative PCR and immunohistochemical assays. The effects of CEMIP on the proliferation, migration and invasion of breast cancer cells were determined through colony formation assay, flow cytometry (FCM) assay, wound healing assay and transwell assay, respectively. Additionally, the downstream targets of CEMIP were examined by Immunoblot assays. RESULTS CEMIP expression was upregulated in both breast cancer tissues and cell lines. Patients with higher CEMIP expression displayed shorter survival time than those with lower expression. In addition, CEMIP knockdown inhibited breast cancer cell proliferation, migration, and invasion in vitro. Our data further confirmed that CEMIP promoted the proliferation and migration of breast cancer cells via GRP78-STAT3 axis. CONCLUSION Our data demonstrated the role of CEMIP in breast cancer was associated with STAT3 signaling pathway. Therefore, CEMIP might act as a possible therapeutic target for breast cancer.

中文翻译：

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细胞迁移诱导透明质酸酶 1 (CEMIP) 激活 STAT3 通路以促进乳腺癌细胞增殖和迁移

目的本文旨在评估CEMIP在人类乳腺癌中的作用，并探讨CEMIP在乳腺癌进展中的潜在机制。方法我们通过定量 PCR 和免疫组织化学分析检测了 CEMIP 在乳腺癌组织和细胞系中的表达水平。CEMIP 对乳腺癌细胞增殖、迁移和侵袭的影响分别通过集落形成试验、流式细胞术 (FCM) 试验、伤口愈合试验和 Transwell 试验确定。此外，CEMIP 的下游靶标通过免疫印迹分析进行了检查。结果 CEMIP 表达在乳腺癌组织和细胞系中均上调。CEMIP 表达较高的患者比表达较低的患者显示出更短的生存时间。此外，CEMIP 敲低抑制了乳腺癌细胞的增殖、迁移和体外侵袭。我们的数据进一步证实 CEMIP 通过 GRP78-STAT3 轴促进乳腺癌细胞的增殖和迁移。结论 我们的数据表明 CEMIP 在乳腺癌中的作用与 STAT3 信号通路有关。因此，CEMIP 可能作为乳腺癌的治疗靶点。