Abstract A high-performance liquid chromatography-electrochemical detection method was developed to determine the concentration of homocysteine and cysteine in the diabetes mellitus Sprague-Dawley male rats’ cortex and hippocampus. The cortex and hippocampus of control and diabetes model rats were collected. After pretreatment, the analysis of total homocysteine and cysteine were performed on a C8 column. Under the optimized analytical conditions, the linear correlation coefficients were higher than 0.999 in the concentration range from 0.148 to 22.193 μmol.L−1 with the limit of quantitation of 0.083 μmol.L−1. The extraction recoveries of Hcy and Cys from spiked samples were 90.87–100.25%, and the relative standard deviations (RSD %) were 1.56–9.13%. The established method is simple, accurate, sensitive, and suitable for the separation and determination of Hcy and Cys in biological samples. Compared with the control group, the concentrations of Hcy and Cys in the diabetes group were different in the cortex. The difference suggests that changes in the levels of the two substances may be associated with complications of diabetes mellitus. Graphical Abstract

中文翻译：

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HPLC-ECD法测定糖尿病大鼠脑中两种相关氨基硫醇的研究

摘要 建立了一种高效液相色谱-电化学检测方法，用于测定糖尿病雄性大鼠皮层和海马中同型半胱氨酸和半胱氨酸的浓度。收集对照和糖尿病模型大鼠的皮质和海马体。预处理后，在 C8 柱上进行总同型半胱氨酸和半胱氨酸的分析。在优化的分析条件下，线性相关系数在0.148~22.193 μmol.L-1的浓度范围内均高于0.999，定量限为0.083 μmol.L-1。加标样品中 Hcy 和 Cys 的提取回收率为 90.87-100.25%，相对标准偏差 (RSD%) 为 1.56-9.13%。建立的方法简单、准确、灵敏、适用于生物样品中Hcy和Cys的分离测定。与对照组相比，糖尿病组的Hcy和Cys在皮质中的浓度不同。这种差异表明这两种物质水平的变化可能与糖尿病并发症有关。图形概要