Abstract Veterinary drugs are widely used in animal husbandry to prevent and treat animal diseases, to promote animal growth, and to improve the conversion rate of feed. However, the use of veterinary drugs in food-producing animals may leave residues of the parent compounds, and/or their metabolites in food products resulting in harmful effects to humans. To ensure food safety, sensitive and effective analytical methods have been developing rapidly. So it is necessary to make a systematic summary of detection methods of veterinary drug residues. Sample preparation methods and analytical techniques are the two main parts of analyzing veterinary drug residues which were described in detail. Sample preparation methods, such as solid-phase extraction, liquid–liquid extraction, immunoaffinity chromatography, and QuEChERS, were summarized by the specific examples. The three types of methods, microbial, immunological, and physicochemical methods, for screening or determination were described, including microbial inhibition, enzyme-linked immunosorbent assay, colloidal gold immunoassay, fluorescence polarization immunoassay, biosensor technology, application of quantum dots, capillary electrophoresis, liquid chromatography, and liquid chromatography-mass spectrometry. In addition, the advantages and shortcomings of methods were also described. The challenges and prospects were discussed for the analysis of veterinary drug residues in animal-derived foods. Graphical Abstract

中文翻译：

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动物源性食品中兽药残留量的测定：样品制备方法和分析技术

摘要 兽药在畜牧业中广泛用于防治动物疾病，促进动物生长，提高饲料转化率。然而，在食用动物中使用兽药可能会在食品中留下母体化合物和/或其代谢物的残留物，从而对人类产生有害影响。为了确保食品安全，灵敏有效的分析方法得到了迅速发展。因此有必要对兽药残留检测方法进行系统总结。样品制备方法和分析技术是分析兽药残留的两个主要部分，其中详细描述了。样品制备方法，如固相萃取、液-液萃取、免疫亲和色谱和 QuEChERS，通过具体的例子进行了总结。描述了用于筛选或测定的微生物、免疫学和物理化学方法三类方法，包括微生物抑制、酶联免疫吸附测定、胶体金免疫测定、荧光偏振免疫测定、生物传感器技术、量子点应用、毛细管电泳、液相色谱法和液相色谱-质谱法。此外，还描述了方法的优缺点。讨论了动物源性食品中兽药残留分析的挑战和前景。图形概要 酶联免疫吸附测定、胶体金免疫测定、荧光偏振免疫测定、生物传感器技术、量子点应用、毛细管电泳、液相色谱、液相色谱-质谱。此外，还描述了方法的优缺点。讨论了动物源性食品中兽药残留分析的挑战和前景。图形概要 酶联免疫吸附测定、胶体金免疫测定、荧光偏振免疫测定、生物传感器技术、量子点应用、毛细管电泳、液相色谱、液相色谱-质谱。此外，还描述了方法的优缺点。讨论了动物源性食品中兽药残留分析的挑战和前景。图形概要 讨论了动物源性食品中兽药残留分析的挑战和前景。图形概要 讨论了动物源性食品中兽药残留分析的挑战和前景。图形概要