ABSTRACT Previously, we reported that endometrial stromal (ES) and endometrial epithelial (EE) cells did not attach to tenascin C, indicating the absence of active integrin α9β1 on the surface of mouse ES and EE cells. However, that study used recombinant tenascin C without fibronectin (FN) type III repeats interacting with integrin heterodimers. Therefore, we re-evaluated the presence of integrin α9β1 actively functioning on the surface of mouse ES and EE cells using full-length native tenascin C with FN type III repeats. The functionality of integrin α9β1 was confirmed using attachment and antibody inhibition assays. Both mouse ES and EE cells showed significantly increased adhesion to native tenascin C, and functional blocking of integrin α9β1 significantly inhibited adhesion to native tenascin C. These results demonstrate that the integrin α9 and β1 subunits function as active heterodimers on the plasma membrane of mouse ES and EE cells, respectively.

中文翻译：

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整合素异源二聚体α9β1在小鼠子宫内膜间质和上皮细胞表面的定位

摘要以前，我们报道子宫内膜基质 (ES) 和子宫内膜上皮 (EE) 细胞没有附着在生腱蛋白 C 上，表明小鼠 ES 和 EE 细胞表面不存在活性整合素 α9β1。然而，该研究使用了没有与整合素异二聚体相互作用的纤连蛋白 (FN) III 型重复序列的重组生腱蛋白 C。因此，我们使用具有 FN III 型重复序列的全长天然生腱蛋白 C 重新评估了整合素 α9β1 在小鼠 ES 和 EE 细胞表面积极发挥作用的存在。使用附着和抗体抑制试验证实了整联蛋白 α9β1 的功能。小鼠 ES 和 EE 细胞对天然生腱蛋白 C 的粘附显着增加，并且整合素 α9β1 的功能阻断显着抑制了对天然生腱蛋白 C 的粘附。