Pathophysiological acidic-pH hinders the dental biomaterial-based pulp-dentin regeneration. Bioactive glass (BG) synthesized by sol-gel methods had shown odontogenic and pulp regeneration potential. However, the pathophysiological acidic-pH neutralizing potential of BG has not been tested yet. In this study, we aimed to design mesoporous BG-nanoparticles by a well-established sol-gel method and test its odontogenic and acidic-pH neutralizing potential. BG-nanoparticles were synthesized and further characterized by SEM, EDS, XRD, and FTIR. Mono-dispersed and spherical mesoporous BG-nanoparticles with size 300–500 nm were successfully fabricated. Effect of BG ionic extraction in DMEM with 0.1–2.5 g/L BG concentrations on proliferation and odontogenic differentiation of stem cells from human exfoliated deciduous teeth (SHED) was analyzed. All the BG ionic extractions did not affect SHEDs proliferation at early time points (day 1 and 3). BG ionic extraction 0.5 g/L robustly enhanced odontogenic differentiation of SHEDs, as shown by the expression pattern of ALP, Col1, DSPP, and matrix mineralization results. The chemical composition of the BG ionic extraction-induced mineralized matrix resembled natural dentin. BG-nanoparticles/alginate paste neutralized the butyric acid solution (pH 5.4) and buffered within pH 8.3 for a month. Our findings showed the odontogenic and pathophysiological acidic-pH neutralizing potential of BG-nanoparticles, indicating its possible application in pulp-dentin regeneration under pathophysiologically challenged acidic environment.

病理生理酸性pH值会阻碍基于牙科生物材料的牙髓-牙本质再生。通过溶胶-凝胶法合成的生物活性玻璃（BG）具有牙源性和牙髓再生潜力。但是，尚未测试BG的病理生理酸性pH值。在这项研究中，我们旨在通过一种完善的溶胶-凝胶法设计中孔BG纳米颗粒，并测试其牙源性和酸性pH中和潜力。合成了BG纳米粒子，并通过SEM，EDS，XRD和FTIR对其进行了进一步表征。成功制备了尺寸为300–500 nm的单分散球形中孔BG纳米颗粒。分析了在BMEM浓度为0.1–2.5 g / L的DMEM中BG离子提取对人脱落乳牙（SHED）干细胞增殖和成牙分化的影响。在早期时间点（第1天和第3天），所有BG离子提取均不影响SHED的增殖。BG离子提取0.5 g / L可以显着增强SHED的牙源性分化，如ALP，Col1，DSPP和基质矿化结果的表达模式所示。BG离子提取诱导的矿化基质的化学组成类似于天然牙本质。BG-纳米颗粒/藻酸盐糊剂中和了丁酸溶液（pH 5.4），并在pH 8.3内缓冲了一个月。我们的发现表明，BG纳米颗粒具有牙源性和病理生理学酸性pH值中和潜力，表明其可能在病理生理挑战性酸性环境下在牙髓-牙本质再生中的应用。5 g / L强有力地增强了SHED的牙源性分化，如ALP，Col1，DSPP和基质矿化结果的表达模式所示。BG离子提取诱导的矿化基质的化学组成类似于天然牙本质。BG-纳米颗粒/藻酸盐糊剂中和了丁酸溶液（pH 5.4），并在pH 8.3内缓冲了一个月。我们的发现表明，BG纳米颗粒具有牙源性和病理生理学酸性pH值中和潜力，表明其可能在病理生理挑战性酸性环境下在牙髓-牙本质再生中的应用。5 g / L强有力地增强了SHED的牙源性分化，如ALP，Col1，DSPP和基质矿化结果的表达模式所示。BG离子提取诱导的矿化基质的化学组成类似于天然牙本质。BG-纳米颗粒/藻酸盐糊剂中和了丁酸溶液（pH 5.4），并在pH 8.3内缓冲了一个月。我们的发现表明，BG纳米颗粒具有牙源性和病理生理学酸性pH值中和潜力，表明其可能在病理生理挑战性酸性环境下在牙髓-牙本质再生中的应用。BG-纳米颗粒/藻酸盐糊剂中和了丁酸溶液（pH 5.4），并在pH 8.3内缓冲了一个月。我们的发现表明，BG纳米颗粒具有牙源性和病理生理学酸性pH值中和潜力，表明其可能在病理生理挑战性酸性环境下在牙髓-牙本质再生中的应用。BG-纳米颗粒/藻酸盐糊剂中和了丁酸溶液（pH 5.4），并在pH 8.3内缓冲了一个月。我们的发现表明，BG纳米颗粒具有牙源性和病理生理学酸性pH值中和潜力，表明其可能在病理生理挑战性酸性环境下在牙髓-牙本质再生中的应用。