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Differentially expressed homologous genes reveal interspecies differences of Paragonimus proliferus based on transcriptome analysis
Helminthologia ( IF 1.1 ) Pub Date : 2020-08-05 , DOI: 10.2478/helm-2020-0029
S H Li 1 , S D Li 1 , H J Li 2 , J Y Li 2 , J J Xu 2 , G J Chang 2 , L J Yang 1 , W Q Wang 1 , Y L Zhang 2 , Z Q Ma 1 , S M He 1 , W L Wang 1 , H L Huang 2
Affiliation  

Summary Paragonimus proliferus (P. proliferus), one of 46 Paragonimus species registered in the National Center for Biotechnology Information database, may be much more widely distributed in Southeast Asia than previously thought, as its reported natural foci have increased in the past decades. However, very little is known about its molecular biology, especially at the transcriptome level. For the first time, the transcriptome of this species was sequenced and compared with four other common Paragonimus species, namely Paragonimus skrjabini, Paragonimus kellicotti, Paragonimus miyazakii, and Paragonimus westermani, to predict homologous genes and differentially expressed homologous genes to explore interspecies differences of Paragonimus proliferus. A total of 7393 genes were found to be significantly differentially expressed. Of these, 49 were considered to be core genes because they were differentially expressed in all four comparison groups. Annotations revealed that these genes were related mainly to “duplication, transcription, or translation”, energy or nutrient metabolism, and parasitic growth, proliferation, motility, invasion, adaptation to the host, or virulence. Interestingly, a majority (5601/7393) of the identified genes, and in particular the core genes (48/49), were expressed at lower levels in P. proliferus. The identified genes may play essential roles in the biological differences between Paragonimus species. This work provides fundamental background information for further research into the molecular biology of P. proliferus.

中文翻译:

基于转录组分析的差异表达同源基因揭示增殖并殖吸虫种间差异

总结 增殖并殖吸虫 (P. proliferus) 是在国家生物技术信息中心数据库中注册的 46 种并殖吸虫之一,在东南亚的分布可能比以前想象的要广泛得多,因为其报告的自然疫源地在过去几十年中有所增加。然而,对其分子生物学知之甚少,尤其是在转录组水平。首次对该物种的转录组进行测序,并与其他四种常见的并殖吸虫物种(Paragonimus skrjabini、Paragonimus kellicotti、Paragonimus miyazakii和Paragonimus westermani)进行比较,预测同源基因和差异表达的同源基因,探索并殖吸虫的种间差异增殖。共发现7393个基因显着差异表达。这些,49个被认为是核心基因,因为它们在所有四个对照组中都有差异表达。注释显示,这些基因主要与“复制、转录或翻译”、能量或营养代谢以及寄生生长、增殖、运动、入侵、对宿主的适应或毒力有关。有趣的是,大多数 (5601/7393) 鉴定的基因,特别是核心基因 (48/49),在 P. proliferus 中的表达水平较低。鉴定出的基因可能在并殖吸虫物种之间的生物学差异中起重要作用。这项工作为进一步研究 P. proliferus 的分子生物学提供了基础背景信息。注释显示,这些基因主要与“复制、转录或翻译”、能量或营养代谢以及寄生生长、增殖、运动、入侵、对宿主的适应或毒力有关。有趣的是,大多数 (5601/7393) 鉴定的基因,特别是核心基因 (48/49),在 P. proliferus 中的表达水平较低。鉴定出的基因可能在并殖吸虫物种之间的生物学差异中起重要作用。这项工作为进一步研究 P. proliferus 的分子生物学提供了基础背景信息。注释显示,这些基因主要与“复制、转录或翻译”、能量或营养代谢以及寄生生长、增殖、运动、入侵、对宿主的适应或毒力有关。有趣的是,大多数 (5601/7393) 鉴定的基因,特别是核心基因 (48/49),在 P. proliferus 中的表达水平较低。鉴定出的基因可能在并殖吸虫物种之间的生物学差异中起重要作用。这项工作为进一步研究 P. proliferus 的分子生物学提供了基础背景信息。大多数 (5601/7393) 鉴定的基因,特别是核心基因 (48/49),在 P. proliferus 中以较低水平表达。鉴定出的基因可能在并殖吸虫物种之间的生物学差异中起重要作用。这项工作为进一步研究 P. proliferus 的分子生物学提供了基础背景信息。大多数 (5601/7393) 鉴定的基因,特别是核心基因 (48/49),在 P. proliferus 中以较低水平表达。鉴定出的基因可能在并殖吸虫物种之间的生物学差异中起重要作用。这项工作为进一步研究 P. proliferus 的分子生物学提供了基础背景信息。
更新日期:2020-08-05
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