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Genetic characterization of hydatid cysts of different intermediate hosts
Helminthologia ( IF 1.1 ) Pub Date : 2020-08-05 , DOI: 10.2478/helm-2020-0031
W M Mousa 1 , A M Abdel-Wahab 1 , M El-Gameel Sohila 1 , O A Mahdy 1
Affiliation  

Summary Cystic echinococcosis is an important cosmopolitan parasitic zoonosis that causes public health and economic problems in Egypt. The present study was undertaken to identify genotypes of hydatid cyst (HC) DNA isolated from different animal isolates and to identify the genotype of secondary hydatid cysts (HCs) developed in rabbits experimentally infected with camel HC for detection of any genetic mutation. In the present study, we extracted DNA from the germinal layers of 8 HCs collected from 3 camels, 1 cattle, 1 sheep and 3 donkeys in addition to 3 secondary HCs collected from rabbits experimentally infected with camel HC. PCR amplification of the ITS1 gene of all examined samples showed an amplified DNA band at 1115 bp. The partial nucleotide sequences of the ITS1 gene of all isolates were aligned and compared with the reference sequences of the genotypes G1–G8 in GenBank. The camel and rabbit samples were identified as Echinococcus canadensis genotype 6 (G6), while the cattle and sheep samples belonged to E. granulosus sensu stricto (G1). The donkey isolates belonged to E. equines (G4). Alignment of the ITS1 partial nucleotide sequences of the camel HCs and rabbit secondary HCs isolates with the G6 partial nucleotide sequence in GenBank was performed. Both camel HCs and rabbit secondary HCs isolates exhibited the same sequence identity matrix, which indicated the absence of mutation in the rabbit secondary HCs. It can be concluded that camel and rabbit samples were identified as E. canadensis (G6), the cattle and sheep samples belonged to E. granulosus sensu stricto (G1) and donkey isolates belonged to E. equines (G4). No mutation occurred during HCs transmission from camel to rabbit.

中文翻译:

不同中间宿主包虫包囊的遗传特征

小结 囊性棘球蚴病是一种重要的世界性寄生虫人畜共患病,在埃及引起公共卫生和经济问题。本研究旨在鉴定从不同动物分离物中分离的包虫胞囊 (HC) DNA 的基因型,并鉴定在用骆驼 HC 实验感染的兔子中发育的继发包虫胞囊 (HC) 的基因型,以检测任何基因突变。在本研究中,我们从 3 只骆驼、1 头牛、1 只绵羊和 3 头驴收集的 8 种 HCs 的生发层中提取 DNA,此外还有从实验感染骆驼 HC 的兔子收集的 3 种次要 HCs。所有检查样品的 ITS1 基因的 PCR 扩增显示在 1115 bp 处有扩增的 DNA 带。对所有分离株的 ITS1 基因的部分核苷酸序列进行比对,并与 GenBank 中 G1-G8 基因型的参考序列进行比对。骆驼和兔子样本被鉴定为加拿大棘球绦虫基因型 6 (G6),而牛和羊样本属于 E. granulosus sensu stricto (G1)。驴分离株属于 E. equines (G4)。将骆驼 HCs 和兔二级 HCs 分离物的 ITS1 部分核苷酸序列与 GenBank 中的 G6 部分核苷酸序列进行比对。骆驼 HCs 和兔次级 HCs 分离物都表现出相同的序列同一矩阵,这表明兔次级 HCs 中不存在突变。可以得出结论,骆驼和兔子样品被鉴定为E. canadensis (G6),牛和羊样品属于E. canadensis (G6)。狭粒细粒 (G1) 和驴分离株属于 E. equines (G4)。HCs从骆驼到兔子的传播过程中没有发生突变。
更新日期:2020-08-05
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