Seaweeds contain many valuable compounds that can be used in the food industry. Carrageenan is a polysaccharide which has been extracted from seaweed for centuries and is used as a texturizer in food and non-food products. However, seaweeds contain compounds other than carrageenan, such as proteins, which could also be extracted. This extraction should be done without compromising the industrial scale carrageenan extraction yield and quality. This study aimed at up-stream protein extraction from red seaweed Eucheuma denticulatum by using of an optimized enzyme-assisted extraction, including of an aqueous/enzymatic treatment followed by alkaline extraction, and then the commercial carrageenan extraction. The protein extraction efficiency of four enzymes was evaluated including Celluclast^® 1.5L, Shearzyme^® 500 L, Alcalase^® 2.4 L FG and Viscozyme^® L at a concentration of 0.0, 0.1, 0.2 and 0.4% (w/w). To avoid detrimental effects on carrageenan, all the experiments were performed at pH 7 at room temperature. The results showed that 0.2% w/w Alcalase^® or Viscozyme^® added individually achieved the highest protein extraction efficiencies (59 and 48%, respectively) at pH 7 and room temperature (p < 0.05). Determination of the most common carrageenan quality parameters indicated that using any of these enzymes had no negative effect on the carrageenan yield and quality.

中文翻译：

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从商业菠菜（Eucheuma denticulatum）中提取蛋白质和角叉菜胶的质量及其质量。

海藻含有许多可用于食品工业的有价值的化合物。角叉菜胶是一种从海藻中提取出来的多糖，已有数百年历史，被用作食品和非食品产品中的纹理化剂。但是，海藻还含有角叉菜胶以外的其他化合物，例如蛋白质，也可以提取。该提取应在不损害工业规模的角叉菜胶提取产率和质量的情况下进行。这项研究旨在通过使用优化的酶辅助提取方法从红海藻Eucheuma denticulatum上游提取蛋白，包括先进行水/酶处理再进行碱提取，然后再进行商业角叉菜胶提取。的四种酶蛋白提取效率进行评价，包括的Celluclast ^®1.5L，SHEARZYME ^® 500 L，碱性蛋白酶^® 2.4升FG和VISCOZYME ^® L的在0.0，0.1，0.2和0.4％（浓度瓦特/瓦特）。为了避免对角叉菜胶的有害影响，所有实验均在室温下于pH 7下进行。结果表明，0.2％瓦特/瓦特的Alcalase ^®或VISCOZYME ^®分别添加在pH 7和室温下（达到的最高蛋白质提取效率（分别为59和48％），p <0.05）。确定最常见的角叉菜胶质量参数表明，使用这些酶中的任何一种对角叉菜胶的产量和质量均无负面影响。