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Citrus CsACD2 is a target of Candidatus Liberibacter asiaticus in Huanglongbing disease.
Plant Physiology ( IF 6.5 ) Pub Date : 2020-10-01 , DOI: 10.1104/pp.20.00348
Zhiqian Pang 1 , Li Zhang 2, 3 , Gitta Coaker 4 , Wenbo Ma 5 , Sheng-Yang He 2, 3 , Nian Wang 6
Affiliation  

Citrus Huanglongbing (HLB), caused by Candidatus Liberibacter asiaticus (Las), is one of the most destructive citrus diseases worldwide, yet how Las causes HLB is poorly understood. Here we show that a Las-secreted protein, SDE15 (CLIBASIA_04025), suppresses plant immunity and promotes Las multiplication. Transgenic expression of SDE15 in Duncan grapefruit (Citrus x paradisi) suppresses the hypersensitive response induced by Xanthomonas citri ssp. citri (Xcc) and reduces the expression of immunity-related genes. SDE15 also suppresses the hypersensitive response triggered by the Xanthomonas vesicatoria effector protein AvrBsT in Nicotiana benthamiana, suggesting that it may be a broad-spectrum suppressor of plant immunity. SDE15 interacts with the citrus protein CsACD2, a homolog of Arabidopsis (Arabidopsis thaliana) ACCELERATED CELL DEATH 2 (ACD2). SDE15 suppression of plant immunity is dependent on CsACD2, and overexpression of CsACD2 in citrus suppresses plant immunity and promotes Las multiplication, phenocopying overexpression of SDE15. Identification of CsACD2 as a susceptibility target has implications in genome editing for novel plant resistance against devastating HLB.



中文翻译:


柑橘 CsACD2 是黄龙病中 Candidatus Liberibacter asiaticus 的靶标。



柑橘黄龙病 (HLB) 由亚洲自由杆菌 (Las) 引起,是世界范围内最具破坏性的柑橘病害之一,但人们对 Las 是如何引起 HLB 的了解甚少。在这里,我们发现 Las 分泌蛋白 SDE15 (CLIBASIA_04025) 可抑制植物免疫并促进 Las 增殖。邓肯葡萄柚 ( Citrus x paradisi ) 中 SDE15 的转基因表达可抑制由柑橘黄单胞菌 ( Xanthomonas citri ssp) 诱导的过敏反应。 citri ( Xcc ) 并降低免疫相关基因的表达。 SDE15 还抑制本塞姆氏烟草中由泡泡黄单胞菌效应蛋白 AvrBsT 引发的过敏反应,表明它可能是植物免疫的广谱抑制剂。 SDE15 与柑橘蛋白 CsACD2 相互作用,CsACD2 是拟南芥 ( Arabidopsis thaliana ) 加速细胞死亡 2 (ACD2) 的同源物。 SDE15对植物免疫的抑制依赖于CsACD2 ,柑橘中CsACD2的过度表达抑制植物免疫并促进Las增殖,表型复制SDE15的过度表达。将 CsACD2 鉴定为易感性靶点对于基因组编辑对于新型植物对破坏性 HLB 的抗性具有重要意义。

更新日期:2020-10-06
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