The phonotactic response by female crickets is influenced by Juvenile Hormone III, which affects selectivity to the syllable period of the calling song. This pathway is influenced by an inhibitory input in the prothoracic ganglion, possibly chloride‐mediated inhibition. In order to identify potential neurotransmitters involved in such pathway, we performed nanoinjection of five neurotransmitters into the prothoracic ganglion of virgin female Acheta domesticus. Phonotaxis for these females was evaluated before and after injections. All five neurotransmitters that were nanoinjected are known to bind to chloride channels. Nanoinjection of histamine significantly decreased phonotactic selectivity to the syllable period of the calling song while glycine, gamma aminobutyric acid, serotonin and saline controls did not. Octopamine significantly decreased phonotactic responses overall. The effect of histamine was tested further by nanoinjecting the antihistamine pyrilamine into the prothoracic ganglion of older unselected females, which resulted in increased phonotactic selectivity.

中文翻译：

---

将神经递质纳米注射到雌性蟋蟀 Achetadomesticus 的前胸神经节改变了音调选择性

雌性蟋蟀的音调反应受保幼激素 III 的影响，这会影响对鸣叫歌曲音节周期的选择性。该通路受前胸神经节抑制输入的影响，可能是氯介导的抑制。为了识别参与这种途径的潜在神经递质，我们将五种神经递质纳米注射到处女雌性 Achetadomesticus 的前胸神经节中。在注射之前和之后评估这些雌性的光趋性。已知所有五种被纳米注射的神经递质都与氯离子通道结合。组胺的纳米注射显着降低了对呼叫歌曲音节周期的定向选择性，而甘氨酸、γ-氨基丁酸、5-羟色胺和盐水对照则没有。章鱼胺总体上显着降低了音调反应。通过将抗组胺剂吡拉胺纳米注射到未选择的老年雌性的胸前神经节中，进一步测试了组胺的作用，这导致了音选择性的增加。