Jaburetox is a recombinant peptide derived from one of the Canavalia ensiformis urease isoforms. This peptide induces several toxic effects on insects of different orders, including interference on muscle contractility in cockroaches, modulation of UDP‐N‐acetylglucosamine pyrophosphorylase (UAP) and nitric oxide synthase (NOS) activities in the central nervous system of triatomines, as well as activation of the immune system in Rhodnius prolixus. When injected, the peptide is lethal for R. prolixus and Triatoma infestans. Here, we evaluated Jaburetox toxicity to Nauphoeta cinerea cockroaches, exploring the effects on the central nervous system through the activities of UAP, NOS, acid phosphatases (ACP), and acetylcholinesterase (AChE). The results indicated that N. cinerea is not susceptible to the lethal effect of the peptide. Moreover, both in vivo and in vitro treatments with Jaburetox inhibited NOS activity, without modifying the protein levels. No alterations on ACP activity were observed. In addition, the enzyme activity of UAP only had its activity affected at 18 hr after injection. The peptide increased the AChE activity, suggesting a mechanism involved in overcoming the toxic effects. In conclusion, our findings indicate that Jaburetox affects the nitrinergic signaling as well as the AChE and UAP activities and establishes N. cinerea as a Jaburetox‐resistant model for future comparative studies.

中文翻译：

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Jaburetox，一种脲酶衍生的肽：对灰鹦鹉Nauphoeta cinerea的酶促途径的影响。

Jaburetox距离之一衍生的重组肽刀豆脲酶同种型。该肽对不同等级的昆虫产生多种毒性作用，包括干扰蟑螂的肌肉收缩力，调节UDP- N-乙酰氨基葡萄糖焦磷酸化酶（UAP）和一氧化氮合酶（NOS）在中枢神经系统中的神经毒性，以及激活Rhodnius prolixus的免疫系统。当注射时，所述肽是致死为R.蝽和骚扰锥蝽。在这里，我们评估了Jaburetox对灰褐线虫的毒性蟑螂，通过UAP，NOS，酸性磷酸酶（ACP）和乙酰胆碱酯酶（AChE）的活性探索对中枢神经系统的影响。结果表明灰葡萄孢菌对肽的致死作用不敏感。此外，在体内和体外用Jaburetox处理都可以抑制NOS活性，而不会改变蛋白质水平。没有观察到ACP活性的变化。另外，UAP的酶活性仅在注射后18小时受到影响。该肽增加了AChE活性，提示了一种克服毒性作用的机制。总之，我们的发现表明Jaburetox影响硝化能信号传导以及AChE和UAP活性并建立灰质猪笼草 作为耐Jaburetox的模型，用于将来的比较研究。