NMR based clinical metabolomics revealed distinctive serum metabolic profiles in patients with Spondyloarthritis,Magnetic Resonance in Chemistry

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NMR based clinical metabolomics revealed distinctive serum metabolic profiles in patients with Spondyloarthritis
Magnetic Resonance in Chemistry ( IF 1.9 ) Pub Date : 2020-08-12 , DOI: 10.1002/mrc.5083
Latika Gupta ₁ , Anupam Guleria ₂ , Atul Rawat ₂ , Dinesh Kumar ₂ , Amita Aggarwal ₁

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Spondyloarthritis (SpA) is a common rheumatic disorder of the young, marred by delay in diagnosis, and paucity of biomarkers of disease activity. The present study aimed to explore the potential of serum metabolic profiling of patients with SpA to identify biomarker for the diagnosis and assessment of disease activity. The serum metabolic profiles of 81 patients with SpA were compared with that of 86 healthy controls (HCs) using nuclear magnetic resonance (NMR)-based metabolomics approach. Seventeen patients were followed up after 3 months of standard treatment, and paired sera were analyzed for effects of therapy. Comparisons were done using the multivariate partial least squares discriminant analysis (PLS-DA), and the discriminatory metabolic entities were identified based on variable importance in projection (VIP) statistics and further evaluated for statistical significance (p value < 0.05). We found that the serum metabolic profiles differed significantly in SpA as compared with HCs. Compared with HC, the SpA patients were characterized by increased serum levels of amino acids, acetate, choline, N-acetyl glycoproteins, Nα-acetyl lysine, creatine/creatinine, and so forth and decreased levels of low-/very low-density lipoproteins and polyunsaturated lipids. PLS-DA analysis also revealed metabolic differences between axial and peripheral SpA patients. Further metabolite profiles were found to differ with disease activity and treatment in responding patients. The results presented in this study demonstrate the potential of serum metabolic profiling of axial SpA as a useful tool for diagnosis, prediction of peripheral disease, assessment of disease activity, and treatment response.

中文翻译：

基于核磁共振的临床代谢组学揭示了脊柱关节炎患者独特的血清代谢特征

脊柱关节炎 (SpA) 是一种常见的年轻人风湿性疾病，由于诊断延迟和疾病活动性生物标志物缺乏而受到损害。本研究旨在探索 SpA 患者血清代谢谱的潜力，以确定用于诊断和评估疾病活动的生物标志物。使用基于核磁共振 (NMR) 的代谢组学方法将 81 名 SpA 患者的血清代谢特征与 86 名健康对照 (HC) 的血清代谢特征进行比较。17 名患者在标准治疗 3 个月后进行了随访，并分析了配对血清的治疗效果。使用多元偏最小二乘判别分析 (PLS-DA) 进行比较，并且根据预测 (VIP) 统计中的变量重要性确定了歧视性代谢实体，并进一步评估了统计显着性（p 值 < 0.05）。我们发现，与 HCs 相比，SpA 的血清代谢谱显着不同。与HC相比，SpA患者的血清氨基酸、醋酸盐、胆碱、N-乙酰糖蛋白、Nα-乙酰赖氨酸、肌酸/肌酐等水平升高，低/极低密度脂蛋白水平降低和多不饱和脂质。PLS-DA 分析还揭示了中轴和外周 SpA 患者之间的代谢差异。发现进一步的代谢物谱随反应患者的疾病活动和治疗而不同。

更新日期：2020-08-12

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