Isolation of active components of therapeutic plants and discovering molecular mechanisms play a pivotal role in therapy of diabetes. This study aimed to determine the antidiabetic mechanism of an oligosaccharide isolated from Rosa canina (RCO) by measuring the expression of some miRNAs and their targets involved in autophagy. RCO was extracted and characterized by using HPLC and spectroscopic methods. Rin‐5F cells were treated with STZ and RCO alone and in combination. The viability of the cells and the expression of miR‐21, miR‐22, Akt, ATG5, Beclin1, LC3A, and LC3B were analyzed using MTT assay, and qRT‐PCR, respectively. Oligosaccharide fraction could improve the viability of RCO‐treated cells as compared to STZ‐treated cells. Further, the expression of autophagy markers was increased in RCO‐treated diabetic cells compared to STZ‐treated cells. The results indicated that the antidiabetic effects of the oligosaccharide components of R. canina seem to be mediated by modulation of autophagy pathway.

中文翻译：

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从犬蔷薇中分离出的寡糖通过调节自噬途径对 STZ 处理的细胞产生保护作用。


分离治疗植物的活性成分并发现分子机制在糖尿病治疗中发挥着关键作用。本研究旨在通过测量一些 miRNA 及其参与自噬的靶标的表达来确定从犬蔷薇(RCO) 中分离的寡糖的抗糖尿病机制。采用 HPLC 和光谱方法提取并表征 RCO。 Rin-5F 细胞单独或联合使用 STZ 和 RCO 处理。分别使用 MTT 测定和 qRT-PCR 分析细胞的活力以及 miR-21、miR-22、Akt、ATG5、Beclin1、LC3A 和 LC3B 的表达。与 STZ 处理的细胞相比，寡糖部分可以提高 RCO 处理的细胞的活力。此外，与 STZ 处理的细胞相比，RCO 处理的糖尿病细胞中自噬标记物的表达增加。结果表明， R. canina寡糖成分的抗糖尿病作用似乎是通过调节自噬途径介导的。