Expression and functional characterization of odorant-binding protein genes in the endoparasitic wasp Cotesia vestalis,Insect Science

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Expression and functional characterization of odorant-binding protein genes in the endoparasitic wasp Cotesia vestalis
Insect Science ( IF 2.9 ) Pub Date : 2020-08-06 , DOI: 10.1111/1744-7917.12861
Yue-Nan Zhou _{1,

2,

3} , Shuang Xie _{1,

2,

3} , Jia-Ni Chen _{1,

2,

3} , Ze-Hua Wang _{1,

2,

3} , Pei Yang _{1,

2,

3} , Si-Cong Zhou _{1,

2,

3} , Lan Pang _{1,

2,

3} , Fei Li _{1,

2,

3} , Min Shi _{1,

2,

3} , Jian-Hua Huang _{1,

2,

3} , Xue-Xin Chen _{1,

2,

3,

4}

Affiliation

Odorant-binding proteins (OBPs) are crucial in insect's olfactory perception, which participate in the initial step of odorant molecules transporting from the external environment to olfactory receptor neurons. To better understand the roles for OBPs in olfactory perception in Cotesia vestalis, a solitary larval endoparasitoid of diamondback moth, Plutella xylostella, we have comprehensively screened the genome of C. vestalis, and obtained 20 CvesOBPs, including 18 classic OBPs and two minus-C OBPs. Motif-pattern analysis indicates that the motifs of C. vestalis OBPs are highly conserved in Hymenoptera. The results of tissue expression analysis show that five OBPs (CvesOBP1/11/12/14/16) are highly expressed in male antennae, whereas six other OBP genes (CvesOBP7/8/13/17/18/19) are significantly transcriptionally enriched in female antennae. The results of RNA interference experiments for three most highly expressed OBP genes (CvesOBP17/18/19) in female antennae demonstrate that they are likely involved in parasitic processes of female wasps, as the wasps take a longer time to target the hosts when they are knocked down.

中文翻译：

内寄生蜂 Cotesia ventalis 中气味结合蛋白基因的表达和功能表征

气味结合蛋白 (OBP) 在昆虫的嗅觉感知中至关重要，它参与气味分子从外部环境传输到嗅觉受体神经元的初始步骤。为了更好地了解在嗅觉感知OBPs角色绒茧蜂vestalis，一个孤独的幼虫小菜蛾的内寄生蜂小菜蛾，我们已经全面筛选基因组C. vestalis，并获得了20个CvesOBPs，其中包括18个经典OBPs二减-C OBP。基序分析表明C.vestalis的基序OBPs 在膜翅目中高度保守。组织表达分析结果表明，5个OBPs（CvesOBP1/11/12/14/16）在雄性触角中高表达，而其他6个OBP基因（CvesOBP7/8/13/17/18/19）在转录上显着富集在雌性触角。对雌性触角中三个最高表达的 OBP 基因 (CvesOBP17/18/19) 的 RNA 干扰实验结果表明，它们很可能参与了雌性黄蜂的寄生过程，因为当它们被寄生时，黄蜂需要更长的时间来瞄准宿主。被击倒。

更新日期：2020-08-06

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