In mammals, there are a number of kinases, including serine/threonine-protein kinase LATS1, that act as a core kinase of the Hippo pathway and that negatively regulate the Hippo effector protein YAP and its paralog TAZ. Using CRISPR/Cas9 technology, we established a stable LATS1 knockdown (LATS1-KD) iPSC from the MUSIi012-A cell line. The LATS1-KD iPSC MUSIi012-A-3 that was developed maintained both the normal karyotype and the pluripotent phenotype, and retained the ability to differentiate into all three embryonic germ layers.

中文翻译：

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丝氨酸/苏氨酸蛋白激酶LATS1基因编辑的iPSC MUSIi012-A-3的生成。

在哺乳动物中，有许多激酶，包括丝氨酸/苏氨酸蛋白激酶LATS1，它们充当Hippo途径的核心激酶，并负调控Hippo效应蛋白YAP及其旁系TAZ。使用CRISPR / Cas9技术，我们从MUSIi012-A细胞系中建立了稳定的LATS1敲低（LATS1-KD）iPSC。开发的LATS1-KD iPSC MUSIi012-A-3既保持了正常的核型，又保持了多能表型，并保留了分化为所有三个胚芽层的能力。