Pulsatile gonadotropin-releasing hormone (GnRH) secretion is essential for regulating reproductive functions in mammals. GnRH pulses are governed by a neural mechanism that is termed the GnRH pulse generator. In the present study, we investigated the role of central calcitonin receptor (CTR) signaling in the regulation of the GnRH pulse generator activity in ovariectomized goats by administering amylin, an endogenous ligand for CTR, into the lateral ventricle. GnRH pulse generator activity was measured using multiple unit activity (MUA) recordings in the mediobasal hypothalamus. We analyzed changes in the interval of characteristic increases in MUA (MUA volleys). The MUA volley interval shortened immediately after amylin administration, followed by prolonged intervals. Double in situ hybridization for KISS1 (kisspeptin gene) and CALCR (CTR gene) revealed that low expression levels of CALCR were found in the arcuate kisspeptin neurons, which is suggested as the main population of neurons, involved in GnRH pulse generator activity. These results suggest that central amylin-CTR signaling has a biphasic role in the regulation of GnRH pulse generator activity by acting on cells other than the arcuate kisspeptin neurons in goats.

促性腺激素释放激素（GnRH）的分泌对于调节哺乳动物的生殖功能至关重要。GnRH脉冲由称为GnRH脉冲发生器的神经机制控制。在本研究中，我们通过在侧脑室中注射胰岛淀粉样蛋白（CTR的一种内源性配体），研究了降钙素中央受体（CTR）信号在卵巢切除山羊的GnRH脉冲发生器活性调节中的作用。使用下丘脑下丘脑中的多个单位活动（MUA）记录来测量GnRH脉冲发生器的活动。我们分析了MUA（MUA截击）中特征增加间隔的变化。胰岛淀粉样多肽给药后，MUA截击间隔立即缩短，随后间隔延长。双原位杂交KISS1（亲吻促动素基因）和CALCR（CTR基因）揭示的低表达水平CALCR在弧形亲吻促动素的神经元，其被建议作为神经元的主要人口，参与GnRH的脉冲发生器活动被发现。这些结果表明，中央胰岛淀粉样多肽-CTR信号通过作用于山羊的弓形吻肽神经元以外的细胞，在调节GnRH脉冲发生器的活动中具有两相作用。