gCJD is a fatal late-onset neurodegenerative disease linked to mutations in the PRNP gene. We have previously shown that transplantation of neural precursor cells (NPCs), or administration of a nanoformulation of pomegranate seed oil (Nano-PSO, GranaGard), into newborn asymptomatic TgMHu2ME199K mice modeling for E200K gCJD significantly delayed the advance of clinical disease. In the present study, we tested the individual and combined effects of both treatments in older and sick TgMHu2ME199K mice. We show that while transplantation of NPCs at both initial (140 days) and advance clinical states (230 days) arrested disease progression for about 30 days, after which scores rapidly climbed to those of untreated Tgs, administration of Nano-PSO to transplanted TgMHu2ME199K mice resulted in detention of disease advance for 60-80 days, followed by a slower disease progression thereafter. Pathological examinations demonstrated the combined treatment extended the survival of the transplanted NPCs, and also increased the generation of endogenous stem cells. Our results suggest that administration of Nano-PSO may increase the beneficial effects of NPCs transplantation.

中文翻译：

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通过结合NPCs移植和Nano-PSO给药延迟患病TgMHu2ME199K小鼠gCJD加重

gCJD 是一种致命的迟发性神经退行性疾病，与 PRNP 基因突变有关。我们之前已经表明，将神经前体细胞 (NPC) 或石榴籽油纳米制剂 (Nano-PSO，GranaGard) 移植到新生无症状的 TgMHu2ME199K 小鼠中，为 E200K gCJD 建模显着延迟了临床疾病的进展。在本研究中，我们测试了两种治疗对老年和患病 TgMHu2ME199K 小鼠的个体和联合作用。我们表明，虽然在初始（140 天）和晚期临床状态（230 天）移植 NPC 可阻止疾病进展约 30 天，此后分数迅速攀升至未经治疗的 Tgs，但对移植的 TgMHu2ME199K 小鼠施用 Nano-PSO导致疾病提前被拘留60-80天，此后疾病进展较慢。病理检查表明联合治疗延长了移植鼻咽癌的存活时间，也增加了内源性干细胞的产生。我们的结果表明，给予 Nano-PSO 可能会增加 NPCs 移植的有益效果。