Senecavirus A (SVA)-associated vesicular disease (SAVD) has extensively been present in the swine industry during the past years. The mechanisms of SVA-host interactions at the molecular level, subsequent to SVA infection, are unclear. We studied the gene expression profiles of LLC-PK1 cells, with or without SVA infection, for 6 h and 12 h using an RNA-seq technology. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed on differentially expressed genes (DEGs). Immune-related genes and pathways were significantly modified after SVA infection. To confirm the RNA-seq data, 28 important DEGs were selected for RT-qPCR assays. All DEGs exhibited expression patterns consistent with the RNA-seq results. Among them, type I IFNs (including IFN-α and IFN-β) showed the largest upregulation, followed by RSAD2, DDX58, MX1 and the 17 other DEGs. In contrary, ID2 and another 5 DEGs were down-regulated or unchanged. These results indicated that type I IFNs play a critical role in host immune responses against SVA infection at early stage, while other immune-regulated genes directly or indirectly participate in the host immune responses.

在过去的几年中，与Senecavirus A（SVA）相关的水疱病（SAVD）已广泛存在于养猪业。SVA感染后，在分子水平上SVA-宿主相互作用的机制尚不清楚。我们使用RNA-seq技术研究了有或没有SVA感染的LLC-PK1细胞的基因表达谱，分别为6 h和12 h。对差异表达基因（DEG）进行了基因本体论（GO）和《京都基因与基因组百科全书》（KEGG）途径富集分析。SVA感染后，与免疫相关的基因和途径被显着改变。为了确认RNA-seq数据，选择了28个重要的DEG用于RT-qPCR分析。所有DEG均表现出与RNA-seq结果一致的表达模式。其中，I型干扰素（包括IFN-α和IFN-β）显示出最大的上调，其次是RSAD2，DDX58，MX1和其他17个DEG。相反，ID2和另外5个DEG被下调或保持不变。这些结果表明，I型干扰素在早期针对SVA感染的宿主免疫反应中起关键作用，而其他免疫调节基因直接或间接参与宿主免疫反应。