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Lactobacillus rhamnosus GG in a mixed pineapple (Ananas comosus L. Merril) and jussara (Euterpe edulis Martius) beverage and its survival in the human gastrointestinal tract
LWT - Food Science and Technology ( IF 6.0 ) Pub Date : 2020-08-06 , DOI: 10.1016/j.lwt.2020.110028
Bruno de Andrade Pires , Renata Cristina de Almeida Bianchini Campos , John Warnens Canuto , Thainá de Melo Carlos Dias , Eliane Maurício Furtado Martins , Leandro Licursi , Bruno Ricardo de Castro Leite Júnior , Maurilio Lopes Martins

This research aimed to elaborate a mixed beverage containing pineapple and jussara palm fruit and evaluate the viability of Lactobacillus rhamnosus GG (LGG) in the product and also its survival in the human gastrointestinal tract (GIT). In order to evaluate the efficiency of the beverage as a vehicle for LGG, fecal samples from 14 volunteers male were collected before consuming the product, 28 days after intervention, and 42 days after interrupting consumption and they were submitted to LGG count. The mean LGG viability in the beverage was 7.5 log cell/mL (p > 0.05) by flow cytometry, and 6.9 log CFU/mL in the standard plate count. No LGG count was detected in the volunteers feces before consuming the beverage. However, after 28 days of consumption, the mean count of this bacterium in the feces was 7.1 log CFU/g, and 42 days after interrupting consumption, the count had reduced to 4.1 log CFU/g. Thus, the beverage was shown to be feasible as a vehicle for LGG in the GIT of the volunteers, demonstrating the capacity for intestinal colonization.



中文翻译:

混合菠萝(Ananas comosus L. Merril)和jussara(Euterpe edulis Martius)饮料中的鼠李糖乳杆菌GG及其在人胃肠道中的存活

这项研究旨在制作一种包含菠萝和果汁软糖的混合饮料,并评估鼠李糖乳杆菌的生存能力。产品中的GG(LGG)以及其在人胃肠道(GIT)中的存活率。为了评估该饮料作为LGG媒介的效率,在食用该产品之前,干预后28天和中断食用后42天收集了14名志愿者的粪便样本,并将其进行LGG计数。通过流式细胞术,饮料中LGG的平均生存力为7.5 log细胞/ mL(p> 0.05),标准板计数中为6.9 log CFU / mL。饮用饮料之前,志愿者的粪便中未检测到LGG计数。但是,食用28天后,粪便中该细菌的平均计数为7.1 log CFU / g,而在中断食用42天后,该细菌的计数降至4.1 log CFU / g。因此,饮料被证明是自愿者GIT中LGG的载体,

更新日期:2020-08-14
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