当前位置: X-MOL 学术LWT Food Sci. Technol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
GABA mediates NO/cGMP/GSK-3-induced isoflavone accumulation in soybean sprouts
LWT - Food Science and Technology ( IF 6.0 ) Pub Date : 2020-08-06 , DOI: 10.1016/j.lwt.2020.110027
Caifeng Jiao , Yongsheng Liu

The effects of glycogen synthase kinase-3 (GSK-3) on nitric oxide (NO)/guanosine 3′,5′-cyclic monophosphate (cGMP)-activated γ-aminobutiric acid (GABA) accumulation and the role of GABA in isoflavone biosynthesis in response to UV-B exposure in soybean sprouts were investigated. The sprouts were sprayed using donors and inhibitors of cGMP and NO as well as inhibitors of GSK-3 and GABA. It turned out that GSK-3, as a second messenger, mediated NO/cGMP/PKG-induced GABA accumulation by activating diamine oxidase (DAO) under UV-B treatment. GABA mediated NO/cGMP/PKG/GSK-3-stimulated the increment of gene and protein expression and activity of chalcone synthase (CHS) and isoflavone synthase (IFS), and consequently induced the elevation in isoflavone content. Overall, GABA mediates NO/cGMP/PKG/GSK-3-stimulated isoflavone production in response to UV-B exposure in soybean sprouts.



中文翻译:

GABA介导NO / cGMP / GSK-3诱导的大豆异黄酮在豆芽中的积累

糖原合酶激酶3(GSK-3)对一氧化氮(NO)/鸟苷3',5'-环一磷酸(cGMP)活化的γ-氨基丁酸(GABA)积累的影响以及GABA在异黄酮生物合成中的作用响应于UV-B暴露的大豆芽进行了研究。使用供体和cGMP和NO的抑制剂以及GSK-3和GABA的抑制剂喷洒芽苗。事实证明,GSK-3作为第二信使,通过在UV-B处理下激活二胺氧化酶(DAO)介导NO / cGMP / PKG诱导的GABA积累。GABA介导的NO / cGMP / PKG / GSK-3-刺激了查尔酮合酶(CHS)和异黄酮合酶(IFS)的基因和蛋白质表达以及活性的增加,从而诱导了异黄酮含量的增加。总体,

更新日期:2020-09-30
down
wechat
bug