Three hundred and twenty endophytic actinobacterial strains were isolated from psammophytes collected from Taklamakan Desert and identified. Among them, three strains already had been identified as new species of two genera and sixteen isolates showed relatively low 16S rRNA similarities < 98.6% to validly described species. Seventy-five of the isolates were selected as representative strains to screen antibacterial activity and mechanism. Forty-seven strains showed antagonistic activity against at least one of the indicator bacteria. Two Streptomyces strains produced bioactive compounds inducing DNA damage, and two Streptomyces strains produced bioactive compounds with inhibitory activity on protein biosynthesis. Notably, the strain Streptomyces sp. 8P21H-1 that demonstrated both strong antibacterial activity and inhibitory activity on protein biosynthesis was prioritized for exploring new antibiotics. Under the strategy of integrating genetics-based discovery program and MS/MS-based molecular networking, two new streptogramin-type antibiotics, i.e., acetyl-griseoviridin and desulphurizing griseoviridin, along with known griseoviridin, were isolated from the culture broth of strain 8P21H-1. Their chemical structures were determined by HR-MS, and 1D and 2D NMR. Desulphurizing griseoviridin and griseoviridin exhibited antibacterial activities by inhibiting translation.

从塔克拉玛干沙漠采集的沙生植物中分离并鉴定了320株内生放线菌。其中，3个菌株已被鉴定为两个属的新种，16个分离株与有效描述的物种显示出相对较低的16S rRNA相似性< 98.6%。选择其中75株作为代表性菌株来筛选抗菌活性和机制。 47 个菌株对至少一种指示菌表现出拮抗活性。两种链霉菌菌株产生诱导 DNA 损伤的生物活性化合物，两种链霉菌菌株产生对蛋白质生物合成具有抑制活性的生物活性化合物。值得注意的是，菌株链霉菌。 8P21H-1表现出强大的抗菌活性和对蛋白质生物合成的抑制活性，是探索新抗生素的优先选择。在整合基于遗传学的发现计划和基于MS/MS的分子网络的策略下，从菌株8P21H-的培养液中分离出两种新的链阳菌素型抗生素，即乙酰灰绿素和脱硫灰绿素以及已知的灰绿素。 1.它们的化学结构通过 HR-MS、1D 和 2D NMR 确定。脱硫灰绿素和灰绿素通过抑制翻译表现出抗菌活性。