Pyrabactin resistance 1-like proteins (PYLs) are direct receptors of abscisic acid (ABA). For the redundant and polymorphic functions, some members of the PYL family interact with components of other signaling pathways. Here, 253 positive colonies from a maize cDNA library were screened as interacting proteins with the members of ZmPYL family. After sequencing and function annotation, 17 of 28 interaction combinations were verified by yeast two-hybrid (Y2H). The germination potential, taproot length and proline content of a quartet mutant of Arabidopsis PYL genes were significantly deceased comparing to the wild type (WT) under alkaline stress (pH 8.5) and 100 µmol L⁻¹ methyl jasmonate (MeJA) induction. The malondialdehyde (MDA) content was significantly increased. After germinating in darkness, the characteristics of dark morphogenesis of the quartet mutant seedlings were more obvious than those of the WT. The differential expression of the related genes of photomorphogenesis in the mutant was much more than that in the WT. Three light and two JA responsive cis-affecting elements were identified during the promoter sequences of the AtPYL1 and AtPYL2 genes. These results suggested that functional polymorphism has evolved among the members of ZmPYL family. In response to developmental and environmental stimuli, they not only function as direct ABA receptors but also interact with components of other signaling pathways mediated JA, brassinosteroid (BR), auxin, etc., and even directly regulate downstream stress-related proteins. These signaling pathways can interact at various crosstalk points and different levels of gene expression within a sophisticated network.

吡菌素抗性1-like蛋白（PYLs）是脱落酸（ABA）的直接受体。对于冗余和多态功能，PYL家族的某些成员与其他信号通路的组成部分相互作用。在这里，从玉米cDNA文库中筛选出253个阳性菌落作为与ZmPYL家族成员相互作用的蛋白质。经过测序和功能注释后，通过酵母双杂交（Y2H）验证了28种相互作用组合中的17种。与野生型（WT）在碱性胁迫（pH 8.5）和100 µmol L ^-1下相比，拟南芥PYL基因四重突变体的发芽势，主根长度和脯氨酸含量明显降低茉莉酸甲酯（MeJA）诱导。丙二醛（MDA）含量显着增加。在黑暗中发芽后，四倍体突变苗的黑暗形态发生特征比野生型更明显。突变体中光形态发生相关基因的差异表达远高于野生型。在AtPYL1和AtPYL2的启动子序列中鉴定出三个轻和两个JA响应顺式影响因素基因。这些结果表明，功能性多态性已在ZmPYL家族成员之间进化。响应发育和环境刺激，它们不仅充当直接的ABA受体，而且还与介导的JA，油菜素类固醇（BR），生长素等其他信号途径的成分相互作用，甚至直接调节下游与压力相关的蛋白质。这些信号传导途径可以在复杂网络中的各种串扰点和基因表达的不同水平上相互作用。