We isolated fifty-two strains from the marine aquaculture ponds in Malaysia that were evaluated for their lipid production and ammonium tolerance and four isolates were selected as new ammonium tolerant microalgae with high-lipid production: TRG10-p102 Oocystis heteromucosa (Chlorophyceae); TRG10-p103 and TRG10-p105 Thalassiosira weissflogii (Bacillariophyceae); and TRG10-p201 Amphora coffeiformis (Bacillariophyceae). Eicosapentenoic acid (EPA) in three diatom strain was between 2.6 and 18.6 % of total fatty acids, which were higher than in O. heteromucosa. Only A. coffeiformi possessed arachidonic acid. Oocystis heteromucosa naturally grew at high ammonium concentrations (1.4–10 mM), whereas the growth of the other strains, T. weissflogii and A. coffeiformi, were visibly inhibited at high ammonium concentrations (>1.4 mM-NH₄). However, two strains of T. weissflogii were able to grow at up to 10 mM-NH₄ by gradually acclimating to higher ammonium concentrations. The ammonium tolerant strains, especially T. weissflogii which have high EPA contents, were identified as a valuable candidate for biomass production utilizing NH₄-N media, such as ammonium-rich wastewater.

我们从马来西亚海水养殖池塘中分离出 52 个菌株，对其脂质产量和铵耐受性进行了评估，并选择了 4 个分离株作为具有高脂质产量的新型耐铵微藻：TRG10-p102 Oocystis heteromucosa (Chlorophyceae)；TRG10-p103 和 TRG10-p105 Thalassiosira weissflogii (Bacillariophyceae); 和 TRG10-p201 Amphora coffeiformis (Bacillariophyceae)。三种硅藻菌株中的二十碳五烯酸 (EPA) 占总脂肪酸的 2.6% 至 18.6% 之间，高于O.heteromucosa。只有A. coffeiformi拥有花生四烯酸。异黏膜卵囊藻在高铵浓度 (1.4–10 mM) 下自然生长，而其他菌株T. weissflogii和A. coffeiformi的生长在高铵浓度 (>1.4 mM-NH ₄ ) 下明显受到抑制。然而，通过逐渐适应更高的铵浓度，T.weissflogii 的两个菌株能够在高达 10 mM-NH _{4 的条件}下生长。耐铵菌株，尤其是具有高 EPA 含量的T.weissflogii，被鉴定为利用 NH ₄ -N 介质（如富含铵的废水）生产生物质的有价值的候选物。