Abstract Improved plant performance under salt and drought stresses has been a challenging task to the plant due to the complexity and multitude of genes that govern them. We describe here, isolation and characterization of an aldehyde dehydrogenase gene (BrALDH7B2) that encodes an antiquitin isolated from Brassica rapa. Overexpression of the antiquitin gene in tobacco conferred tolerance to salt and drought stresses at the seedling stage as reflected by fresh weight, root length, and chlorophyll and carotenoid contents. The improved physiological characteristics observed in the transgenics may be ascribed to better PIabs, Pn, gs, WUE and E leading to better endurance under salt stress. The overexpression of BrALDH7B2 improved photosynthetic performance accompanied by reduced ROS formation followed by repressed cell-death in roots as determined by intracellular accumulation of H2DCF-DA and PI during salt stress in transgenic plants. Under salt stress, ALDH expressing tobacco transgenic plants showed activation of abiotic stress-responsive genes and vigorous ROS-scavenging system at the molecular level. Altogether, BrALDH7B2 represents a good candidate gene for orchestrating stress responses and with potential for engineering stress tolerance in different crops. To the best of our knowledge, this is the first report on functional study of BrALDH gene in the heterologous system tobacco with enhanced photosynthetic performance under salt stress.

中文翻译：

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BrALDH7B2 基因的异位表达编码芸苔中的古蛋白赋予烟草对非生物胁迫的耐受性并改善烟草在盐胁迫下的光合性能

摘要 由于控制植物的基因的复杂性和众多，在盐和干旱胁迫下改善植物性能一直是植物面临的一项具有挑战性的任务。我们在这里描述了一种醛脱氢酶基因 (BrALDH7B2) 的分离和表征，该基因编码从芸苔中分离的古素。Antiquitin 基因在烟草中的过度表达赋予了幼苗期对盐和干旱胁迫的耐受性，如鲜重、根长以及叶绿素和类胡萝卜素含量所反映。在转基因中观察到的改善的生理特征可能归因于更好的 PIabs、Pn、gs、WUE 和 E，从而导致更好的盐胁迫耐受性。BrALDH7B2 的过表达改善了光合性能，伴随着 ROS 形成的减少，随后根中的细胞死亡受到抑制，这由转基因植物中盐胁迫期间 H2DCF-DA 和 PI 的细胞内积累所确定。在盐胁迫下，表达 ALDH 的烟草转基因植物在分子水平上表现出非生物胁迫响应基因的激活和强大的 ROS 清除系统。总而言之，BrALDH7B2 代表了一个很好的候选基因，用于协调胁迫反应，并具有在不同作物中工程胁迫耐受的潜力。据我们所知，这是对盐胁迫下光合性能增强的异源系统烟草中BrALDH基因功能研究的首次报道。在盐胁迫下，表达 ALDH 的烟草转基因植物在分子水平上表现出非生物胁迫响应基因的激活和强大的 ROS 清除系统。总而言之，BrALDH7B2 代表了一个很好的候选基因，用于协调胁迫反应，并具有在不同作物中工程胁迫耐受性的潜力。据我们所知，这是对盐胁迫下光合性能增强的异源系统烟草中BrALDH基因功能研究的首次报道。在盐胁迫下，表达 ALDH 的烟草转基因植物在分子水平上表现出非生物胁迫响应基因的激活和强大的 ROS 清除系统。总而言之，BrALDH7B2 代表了一个很好的候选基因，用于协调胁迫反应，并具有在不同作物中工程胁迫耐受性的潜力。据我们所知，这是对盐胁迫下光合性能增强的异源系统烟草中BrALDH基因功能研究的首次报道。