Abstract In this study, silica@chitosan-glutaraldehyde (Si@Cs-G) was synthesized as a novel adsorbent for extraction of Penicillin G (PG) from the synthetic and real samples followed by HPLC determination. The synthesized adsorbents were characterized by the scanning electron microscopy (SEM), X-ray diffraction (XRD), fourier transform infrared (FTIR), dynamic light scattering (DLS), transmission electron microscopy (TEM) and nitrogen adsorption–desorption techniques. The factors influencing the extraction efficiency including pH, sorbent dose, extraction time, extraction solvent type and its volume were investigated and optimized. Under the optimal conditions (sorbent dosage: 25 mg, desorption solvent (acetonitrile) with volume of 0.75 mL; pH: 6 and extraction time: 50 min), the Si@Cs-G demonstrated high efficiency and linearity (R2 > 0.999) with the concentration of penicillin G ranging from 1 to 300 μg L−1. Extraction recovery in synthetic samples was 98.977%, with LOD = 0.493 μg L−1, LOQ = 1.638 μg L−1 and RSD

中文翻译：

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一种新型二氧化硅负载壳聚糖/戊二醛作为一种高效吸附剂，用于固相萃取与高效液相色谱联用，用于测定水和废水样品中的青霉素 G

摘要 在本研究中，合成了二氧化硅@壳聚糖-戊二醛 (Si@Cs-G) 作为一种新型吸附剂，用于从合成和实际样品中提取青霉素 G (PG)，然后进行 HPLC 测定。通过扫描电子显微镜 (SEM)、X 射线衍射 (XRD)、傅里叶变换红外 (FTIR)、动态光散射 (DLS)、透射电子显微镜 (TEM) 和氮吸附-解吸技术对合成的吸附剂进行了表征。对影响提取效率的因素包括pH值、吸附剂用量、提取时间、提取溶剂类型及其体积等因素进行了研究和优化。在最佳条件下（吸附剂用量：25 mg，解吸溶剂（乙腈）体积为 0.75 mL；pH：6，萃取时间：50 min），Si@Cs-G 表现出高效率和线性（R2 > 0。999)，青霉素 G 的浓度范围为 1 至 300 μg L-1。合成样品的萃取回收率为 98.977%，LOD = 0.493 μg L-1，LOQ = 1.638 μg L-1 和 RSD