The present study investigates the effect of four microalgal diets in two dietary modes viz. mono diets (Dunaliella salina, Isochrysis galbana, Chaetoceros calcitrans) and combined diet (Isochrysis galbana and Chaetoceros calcitrans) on growth parameters such as morphological development, larvae survival and metamorphosis of Salmacis bicolor species grown under laboratory conditions for a period of 25th days post fertilization. Micoralgal fatty acids compositions was determined by Gas chromatography - mass spectrometry (GC-MS). Significant difference (p < 0.05) in the growth response was observed between the mono and combined diets. Among the mono diets, better growth in terms of survival rate (45.6 ± 1.2%), larvae length (468.1 ± 3.1 μm), post oral arm length (191.5 ± 3.3 μm) and metamorphosis rate (42.41 ± 2.4%) was achived in larvae fed with mono diet: C. calcitrans. Beside, combined diet has promoted high survival rate (62.3 ± 2.1%), larvae length (642.12 ± 3.6 μm), post oral arm length (232.1 ± 2.5 μm), larvae width (410.48 ± 1.8 μm), stomach length (336.8 ± 2.6 μm) and metamorphosis rate (53.8 ± 3.2%) with high significant level (p < 0.05) compared to the mono microalgal diets treatments. The fatty acid analysis revealed high concentration of vaccenic acid (C18:1–12.34 ± 0.11 mg/ml), linoleic acid (C18:2–15.78 ± 0.25 mg/ml) n-6 PUFA and alpha-linolenic acid (C18:3–19.4 ± 0.41 mg/ml) n-3 PUFA levels in combined diet. From the observations of the present study, it was concluded that combined diet using I. galbana and C. calcitrans could be adopted as best candidate microalgal species for promoting large scale production of competent larvae of S. bicolor.

本研究调查了两种饮食方式下四种微藻饮食的效果。单一日粮（杜氏盐藻，等鞭金藻，白僵菌）和混合日粮（等鞭金藻和白僵菌）的生长参数，例如在实验室条件下受精后第25天生长的双色种的形态发育，幼虫存活和变态等。。通过气相色谱-质谱法（GC-MS）测定微唾液酸的脂肪酸组成。显着差异（p 单一饮食和混合饮食之间的生长反应<0.05）。在单一饮食中，在成活率（45.6±1.2％），幼虫长（468.1±3.1μm），口腔后臂长（191.5±3.3μm）和变态率（42.41±2.4％）方面，生长得到了更好的发展。饲喂单一饮食的幼虫：C. calcitrans。此外，综合饮食还提高了成活率（62.3±2.1％），幼虫长（642.12±3.6μm），口腔后臂长（232.1±2.5μm），幼虫宽度（410.48±1.8μm），胃长（336.8±） 2.6μm）和高水平的变态率（53.8±3.2％）（p <0.05）与单微藻饮食治疗相比。脂肪酸分析显示高浓度的痘苗酸（C18：1–12.34±0.11 mg / ml），亚油酸（C18：2–15.78±0.25 mg / ml）n-6 PUFA和α-亚麻酸（C18：3 –19.4±0.41 mg / ml）联合饮食中n-3 PUFA的水平。从本研究的观察结果可以得出结论，使用加尔纳香蕉和钙转钙酵母的联合饮食可以作为最佳候选微藻种类，以促进大规模生产双色链霉菌幼虫。