In this research, by using the metalloligands approach, a new heterometallic–organic framework has been successfully prepared by one-step synthesis of reacting H₄L with metal acetate in water and EtOH mixed solvent, and its chemical formula is {CdNa₂(H₂O)₃[(CoL)₂Na(OH)(H₂O)]}·6H₂O (1, H₄L = 3,3′-((1E,1′E)-(cyclohexane-1,2-diylbis(azanylylidene))bis(methanylylidene))bis(4-hydroxybenzoic acid)). In the biological study, the CCK-8 method was exploited to determine the survival rate of MCF7 breast cancer cells after compound treatment. The plate colony formation test was used to confirm the inhibitory effect of this compound on the proliferation of MCF7 cancer cell. Besides, the Annexin V-FITC/PI assay were performed to evaluate the apoptosis of compound-treated MCF7 cells in flow cytometer, the intracellular ROS level was also measured via Reactive Oxygen Species Assay Kit in this study.

中文翻译：

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基于Co（salen）的金属金属框架：抑制乳腺癌细胞的增殖，迁移和侵袭

在这项研究中，通过使用金属配位体方法，通过一步合成H ₄ L与金属乙酸盐在水和EtOH混合溶剂中的反应，成功地制备了一种新的杂金属-有机骨架，其化学式为{CdNa ₂（H ₂ O）₃ [（CoL）₂ Na（OH）（H ₂ O）]}·6H ₂ O（1，H ₄L ＝ 3，3'-（（（1E，1'E）-（环己烷-1,2-二基双（氮杂亚烷基））双（甲亚烷基））双（4-羟基苯甲酸））。在生物学研究中，CCK-8方法用于确定复合治疗后MCF7乳腺癌细胞的存活率。平板菌落形成试验用于证实该化合物对MCF7癌细胞的增殖的抑制作用。此外，通过流式细胞仪进行膜联蛋白V-FITC / PI测定以评估经化合物处理的MCF7细胞的凋亡，本研究中还通过反应性氧气分析试剂盒测量了细胞内ROS水平。