Colchicine is an alkaloid that used in the relief of acute gout. Three simple, sensitive, selective and rapid methods have been developed for the determination of colchicine in the presence of its alkaline degradation product with good applicability on its pharmaceutical formulation. The first method was based on TLC separation of colchicine and its degradation using chloroform/methanol/conc ammonia (8:2:0.1 v/v/v) as a mobile phase and densitometric detection at 350 nm. The second method was HPLC using kromasil C18 column and a mobile phase of methanol/ammonia (100:1.5 v/v) with UV detection at 246 nm. The third method was spectrophotometry which involved induced dual wavelength method at 245 nm and 261 nm and first derivative of ratio spectra at 302.4 nm. The developed methods were validated according to ICH guidelines and successfully applied for the determination of colchicine in tablets.

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稳定性指示色谱法和紫外分光光度法测定秋水仙碱

秋水仙碱是一种生物碱，可用于缓解急性痛风。已经开发了三种简单，灵敏，选择性和快速的方法，用于在秋水仙碱碱降解产物存在下测定秋水仙碱的方法，并在其药物制剂中具有良好的适用性。第一种方法基于秋水仙碱的薄层色谱分离及其使用氯仿/甲醇/浓氨水（8：2：0.1 v / v / v）作为流动相的降解以及在350 nm处的光密度检测。第二种方法是使用kromasil C18柱和甲醇/氨水（100：1.5 v / v）的流动相进行HPLC并在246 nm处进行UV检测的方法。第三种方法是分光光度法，它涉及在245 nm和261 nm处的诱导双波长法和在302.4 nm处的比率光谱的一阶导数。