Abstract

In recent years, the application of bioengineering methods has attracted special focus because of simplicity, low cost, high efficiency, non-toxicity, and environmental compatibility. The present study is the first report of biosynthesis of silver nanoparticles (SNPs) using Boerhavia elegans (choisy). 2 mL of extract was added to 4 mL of AgNO₃ with concentration of 1 mM. In order to obtain nanoparticles (NPs) with uniform shape and size, factors affecting the synthesis of NPs, such as: pH of reaction, volume of extract, concentration of silver (I), temperature and time of reaction were evaluated and optimized using UV–Vis spectrophotometry. To characterize the NPs, Transmission Electron Microscopy (TEM) and Power X-ray Diffraction (PXRD) and Fourier-Transformed Infrared Spectroscopy techniques were used. Finally, the antimicrobial activities of SNPs were investigated on four and two pathogenic bacteria and fungi using disc diffusion method. The SNPs showed Surface Plasmon Resonance centered at 405 nm. The PXRD pattern and TEM analysis revealed spherical, stable, and uniform SNPs with the average particle size of 15 nm. The antimicrobial activity of the synthesized SNPs showed a significant microbial effect on all clinical isolates especially, bacteria and fungi. These results suggest that such stable and uniform SNPs can be synthesized rapidly and simply for clinical as well as pharmaceutical applications.

Graphic Abstract

中文翻译：

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利用秀丽线虫（Boerhavia elegans）方便，低成本，快速地合成稳定和环保的银纳米颗粒（选择）并研究其抗菌活性

摘要

近年来，生物工程方法的应用由于其简单，低成本，高效率，无毒和环境相容性而引起了人们的特别关注。本研究是首次使用Boerhavia elegans（choisy）生物合成银纳米颗粒（SNP）的报道。将2 mL提取物添加到4 mL AgNO _3中浓度为1 mM。为了获得形状和大小均一的纳米颗粒（NPs），使用UV评估并优化了影响NPs合成的因素，例如：反应的pH值，提取物的体积，银的浓度，反应的温度和时间。 –可见分光光度法。为了表征NP，使用了透射电子显微镜（TEM）和功率X射线衍射（PXRD）以及傅立叶变换红外光谱技术。最后，采用圆盘扩散法研究了SNP对四种和两种病原菌和真菌的抗菌活性。SNP显示中心在405 nm处的表面等离振子共振。PXRD图谱和TEM分析显示球形，稳定且均匀的SNP，平均粒径为15 nm。合成的SNP的抗菌活性对所有临床分离株，尤其是细菌和真菌，均显示出显着的微生物作用。这些结果表明，这种稳定且均匀的SNP可以快速简便地合成用于临床以及药物应用。

图形摘要