Monochasma savatieri Franch. ex Maxim is a valuable medicinal plant that is currently threatened due to human over excavation. Therefore, an efficient plant micropropagation protocol for M. savatieri through seed germination and direct shoot regeneration is described. The effects of different seed germination treatments, explant source on shoot induction, plant growth regulators on shoot multiplication, and survival rate of regenerated plants were examined. The highest M. savatieri seed germination rate was over 90% on Murashige and Skoog (MS) medium supplemented with 0.1 mg L⁻¹ 6-benzylaminopurine (6-BA) and 0.01 mg L⁻¹ α-naphthaleneacetic acid (NAA), with the survival rate reaching 86.0% one-mo after transplanting to soil. Direct shoot proliferation was achieved with two explant types, but the use of nodal stem explants gave best results in comparison to shoot tip explants regarding shoot multiplication frequency and average number of shoots obtained per explant. For nodal segments, medium supplemented with 0.5 mg L⁻¹ 6-BA and 0.2 mg L⁻¹ NAA gave the highest multiplication frequency of 90.0%. Among the various treatments tested for shoot multiplication, the best response occurred on MS medium containing 0.5 mg L⁻¹ 6-BA and 0.5 mg L⁻¹ gibberellin (GA), with the number of shoots per explant reaching 27.6 and the average height of 4.25 cm. To obtain thick, elongated, and good quality buds, 6-BA at 0.1 and 0.5 mg L⁻¹ along with 0.4 mg L⁻¹ GA should be used interchangeably. Four-mo after transplantation, the survival rates were significantly affected by the types and concentrations of plant growth regulators used during plant tissue culture. The most effective combination of plant growth regulators 0.2 mg L⁻¹ was 6-BA and 0.5 g L⁻¹ ABT (mixture composed of 20% α-naphthaleneacetic acid content and 30% indole-3-acetic acid as active ingredients) with a survival rate of 85.5%. The in vitro propagation protocol for M. savatieri reported here will be useful for the rapid and large-scale propagation of this species.

萨瓦蒂（Schatieri） Franch。前马克西姆是一种有价值的药用植物，目前由于人类过度挖掘而受到威胁。因此，描述了通过种子萌发和直接枝条再生用于鼠尾草的有效植物微繁殖方案。考察了不同种子发芽处理，外源来源对芽诱导的影响，植物生长调节剂对芽繁殖以及再生植物成活率的影响。在补充有0.1 mg L ^-1 6-苄基氨基嘌呤（6-BA）和0.01 mg L ^-1的Murashige和Skoog（MS）培养基上，最高的萨瓦分枝杆菌种子发芽率超过90％。α-萘乙酸（NAA）移植到土壤中后，一月生存率达到86.0％。两种类型的外植体均可实现直接的芽增殖，但与茎尖外植体相比，使用节茎外植体在每棵外植体的繁殖倍数和平均芽数方面可提供最佳结果。对于节段，补充有0.5 mg L ^-1 6-BA和0.2 mg L ^-1 NAA的培养基的最高繁殖频率为90.0％。在用于芽繁殖的各种测试方法中，最佳反应发生在含有0.5 mg L ^-1 6-BA和0.5 mg L ^-1的MS培养基上赤霉素（GA），每个外植体的芽数达到27.6，平均高度为4.25厘米。为了获得浓密，细长和优质的芽，应互换使用0.1和0.5 mg L ^-1的6-BA和0.4 mg L ^-1 GA。移植后四个月，成活率明显受植物组织培养过程中所用植物生长调节剂的类型和浓度的影响。植物生长调节剂0.2 mg L ^-1的最有效组合是6-BA和0.5 g L ^-1 ABT（由20％的α-萘乙酸含量和30％的吲哚-3-乙酸含量为有效成分组成的混合物）生存率85.5％。在体外用于传播协议M. savatieri 此处报道的报道对于该物种的快速大规模繁殖将是有用的。