Psychological stress is an important cause to induce various metabolic disorders such as obesity, type II diabetes, and cardiovascular disorders by affecting the visceral adipose tissue. Pathophysiology of these diseases is often accompanied by the hyperactive immune system. The hyperactive immune system causes immune cells to infiltrate in the adipose tissue to increase the severity of metabolic disorders and to affect the levels of stress associated hormones, such as cortisol and serotonin. Cortisol and serotonin, alone or together, could regulate several aspects of the metabolic and immunological deregulations by manipulating the lipid accumulation or adipogenesis in cells. During adipogenesis, macrophages are recruited. Previous reports from the Aich laboratory established the roles of cortisol and serotonin to influence adipogenesis in pre-adipocytes 3T3-L1 in the presence and absence of macrophages. In the current study, we reported the role of macrophage RAW264.7, especially its polarized states, on differentiated murine adipocytes 3T3-L1 in the presence or absence of cortisol and serotonin. The current study also compares the differential role of macrophage recruitment on pre- and differentiated adipocytes.

心理压力是通过影响内脏脂肪组织而引起各种代谢性疾病（例如肥胖症，II型糖尿病和心血管疾病）的重要原因。这些疾病的病理生理学常伴有免疫系统亢进。过度活跃的免疫系统会导致免疫细胞浸润到脂肪组织中，从而增加新陈代谢疾病的严重程度，并影响与压力相关的激素（例如皮质醇和5-羟色胺）的水平。皮质醇和5-羟色胺单独或一起可以通过操纵细胞中脂质的积累或成脂作用来调节代谢和免疫失调的多个方面。在脂肪形成期间，募集巨噬细胞。来自Aich实验室的先前报告确立了皮质醇和5-羟色胺在存在和不存在巨噬细胞的情况下影响前脂肪细胞3T3-L1中脂肪形成的作用。在当前的研究中，我们报道了在存在或不存在皮质醇和5-羟色胺的情况下，巨噬细胞RAW264.7（尤其是其极化状态）对分化的鼠类脂肪细胞3T3-L1的作用。当前的研究还比较了巨噬细胞募集对脂肪细胞和分化前脂肪细胞的不同作用。