Abstract

Expression of the Col2a1 gene involved in collagen synthesis in the blood and esophageal mucus was shown to be reduced in alkaline esophageal burns of the second degree (AEB 2). When melanin was administered, Col2a1 gene expression levels in the blood and esophageal tissues were increased as compared to those in AEB 2. During the histological study, the intensity of deposition of collagen fibers was determined, the esophageal stenosis index was measured, and the effect of melanin on the healing processes was identified: less pronounced formation of collagen fibers in damaged esophagus tissues. It was found that the serum and esophageal mucosa had higher levels of proinflammatory cytokine IL-6 and lower levels of anti-inflammatory cytokine IL-10. At the administration of melanin, we observed normalization of the content of cytokines in comparison with the indicators for AEB 2, which indicates the anti-inflammatory and antifibrotic properties of this substance and suggests the prospect of using melanin as a substance that contributes to the healing of chemically induced esophageal burns without the formation of pathological scars.

中文翻译：

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食管碱性烧伤和黑色素治疗的分子遗传学和细胞学特征

摘要

血液和食管粘液中胶原合成相关的Col2a1基因的表达在第二度碱性食管烧伤（AEB 2）中显示降低。当施用黑色素时，Col2a1与AEB 2相比，血液和食管组织中的基因表达水平增加了。在组织学研究中，确定了胶原纤维的沉积强度，测量了食管狭窄指数，并研究了黑色素对愈合过程的影响。已确定：受损食道组织中胶原纤维的形成不明显。发现血清和食道粘膜具有较高水平的促炎细胞因子IL-6和较低水平的抗炎症细胞因子IL-10。在施用黑色素时，与AEB 2的指标相比，我们观察到细胞因子含量的正常化