Abstract

To clarify a possible role of jasmonate signaling in regulation of the state of stomata under salt stress, the effect of the treatment of Arabidopsis thaliana L. plant leaves (wild type and mutants deficient in jasmonate signaling) with sodium chloride (50–200 mM) and/or methyl jasmonate (200 μM) was studied. A 2–3-h effect of NaCl induced stomatal closure in wild-type plants (Col-0). The most noticeable effect was observed under the influence of 100 mM sodium chloride. At the same time, NaCl treatment of the leaves of the jin1 mutant, deficient by the gene encoding the JIN1/MYC2 transcription factor, had hardly any effect on the state of the stomata. Only a slight decrease in stomatal aperture occurred under the influence of salt in the plants of the coi1 genotype (mutants by the gene encoding the COI1 protein, which is involved in the removal of jasmonate signaling transcriptional factors’ repressors). A 3-h treatment of leaves with 200 μM methyl jasmonate caused a noticeable stomatal closure in wild-type plants but not in jin1 and coi1 mutants. With the combined effect of methyl jasmonate and salt on the leaves of Arabidopsis of three genotypes, a trend towards additional decrease in the stomatal aperture was manifested only in wild-type plants. A conclusion was drawn on the role of jasmonate and its signal transduction system in the regulation of stomatal movements under salt stress.

中文翻译：

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茉莉酸信号成分参与盐胁迫诱导的拟南芥气孔关闭。

摘要

为了阐明茉莉酸信号在盐胁迫下调节气孔状态中的可能作用，用氯化钠（50–200 mM）处理拟南芥植物叶片（野生型和缺乏茉莉酸信号的突变体）的效果和/或茉莉酸甲酯（200μM）进行了研究。NaCl的2–3小时效应导致野生型植物的气孔关闭（Col-0）。在100 mM氯化钠的影响下观察到最明显的效果。与此同时，NaCl处理的叶子的进1突变体，通过编码进1 / MYC2转录因子的基因缺陷，有难以对气孔的状态的任何影响。盐在coi1植物中的气孔孔径仅略有减少基因型（由编码COI1蛋白的基因引起的突变，与茉莉酸酯信号传导转录因子的阻遏物的去除有关）。叶与200μM的茉莉酮酸甲酯的A 3-H处理，在野生型植物但不引起明显的气孔关闭进1和COI1突变体。茉莉酸甲酯和盐对三种基因型拟南芥叶片的联合作用，仅在野生型植物中显示出气孔孔径进一步降低的趋势。得出了茉莉酸及其信号转导系统在盐胁迫下调节气孔运动中的作用的结论。