This work focused on the detection of cortisol on an interdigitated electrode sensor surface using an anti-cortisol antibody. To improve immobilization, antibodies were conjugated with silver nanoparticles and attached to the surface of the sensor. Cortisol interacted in a dose-dependent manner on the antibody-immobilized sensor surface, and current changes were observed. Linear regression analysis was performed by a 3σ calculation, and the limit of detection fell into the range of 0.01 and 0.1 ng/mL. The sensitivity of cortisol was calculated to be 0.01 ng/mL and the sensor discriminated against other hormones, namely norepinephrine and progesterone, with higher selectivity for cortisol. This result represented the selective detection of cortisol with high performance, which can help to determine anxiety disorders.

这项工作的重点是使用抗皮质醇抗体在叉指电极传感器表面检测皮质醇。为了提高固定性，抗体与银纳米颗粒结合并附着在传感器表面。皮质醇在抗体固定的传感器表面上以剂量依赖性方式相互作用，并观察到电流变化。线性回归分析由 3 σ计算，检测限在0.01～0.1 ng/mL范围内。皮质醇的灵敏度计算为 0.01 ng/mL，传感器区分其他激素，即去甲肾上腺素和黄体酮，对皮质醇具有更高的选择性。这一结果代表了对皮质醇的高性能选择性检测，可以帮助确定焦虑症。