The objective of present research work is to design and characterize camptothecin gel using Carbopol-934 for the treatment of epidermoid carcinoma. Optimized herbal gel formulations were evaluated for homogeneity and appearance, viscosity, extrudability, spreadability, drug content, drug release, pH, and in vitro skin cancer activity on A431 cell lines. Mass and Infrared Spectra respectively conforms molecular weight and functional groups present in camptothecin. All the formulations F1 to F5 showed good homogeneity, pH from 6.68 to 6.90, spreadability in the range of 15.81–23.37 gm.cm/s, extrudability 85.51–90.45% w/w, drug content 89.12–96.64%, and in vitro diffusion 88.36–98.40%, respectively. The drug release study showed that all the formulations followed a diffusion-controlled, zero-order release mechanism. Anticancer activity results indicate that camptothecin gel induce cell death in A-439 cells having IC50 48.03 μg and % apoptosis 54.67 ± 4.58. Topical delivery of camptothecin alleviates the side effects caused by systemic chemotherapy; hence, the developed herbal gel formulation can be effectively useful to deliver camptothecin in the treatment of epidermoid carcinoma on A-431 cells.

中文翻译：

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喜树碱凝胶治疗表皮样癌的设计与表征

本研究工作的目的是使用Carbopol-934设计和表征喜树碱凝胶，以治疗表皮样癌。评价了优化的草药凝胶制剂的同质性和外观，粘度，可挤出性，铺展性，药物含量，药物释放，pH值以及在A431细胞系上的体外皮肤癌活性。质谱和红外光谱分别符合喜树碱中存在的分子量和官能团。所有配方F1至F5均显示出良好的均质性，pH在6.68至6.90之间，铺展性在15.81–23.37 gm.cm/s的范围内，可挤出性在85.51–90.45％w / w，药物含量为89.12–96.64％，并且在体外扩散88.36–98.40％。药物释放研究表明，所有制剂均遵循扩散控制的零级释放机制。抗癌活性结果表明喜树碱凝胶诱导具有IC50 48.03μg和凋亡百分比54.67±4.58的A-439细胞死亡。喜树碱的局部给药减轻了全身化疗引起的副作用；因此，开发的草药凝胶制剂可有效用于在喜树碱上治疗A-431细胞上传递喜树碱。