Photocycle of Cyanobacteriochrome TePixJ.,Biochemistry

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Photocycle of Cyanobacteriochrome TePixJ.
Biochemistry ( IF 2.9 ) Pub Date : 2020-08-04 , DOI: 10.1021/acs.biochem.0c00382
Samantha J O Hardman ₁ , Derren J Heyes ₁ , Igor V Sazanovich ₂ , Nigel S Scrutton ₁

Affiliation

Due to the recent advances in X-ray free electron laser techniques, bilin-containing cyanobacteriochrome photoreceptors have become prime targets for the ever-expanding field of time-resolved structural biology. However, to facilitate these challenging studies, it is essential that the time scales of any structural changes during the photocycles of cyanobacteriochromes be established. Here, we have used visible and infrared transient absorption spectroscopy to probe the photocycle of a model cyanobacteriochrome system, TePixJ. The kinetics span multiple orders of magnitude from picoseconds to seconds. Localized changes in the bilin binding pocket occur in picoseconds to nanoseconds, followed by more large-scale changes in protein structure, including formation and breakage of a second thioether linkage, in microseconds to milliseconds. The characterization of the entire photocycle will provide a vital frame of reference for future time-resolved structural studies of this model photoreceptor.

中文翻译：

蓝细菌色素TePixJ的光循环。

由于X射线自由电子激光技术的最新进展，含胆汁的蓝细菌色素感光体已成为时间分辨结构生物学领域不断扩大的主要目标。但是，为了促进这些具有挑战性的研究，必须建立蓝细菌色素光循环过程中任何结构变化的时间尺度。在这里，我们使用了可见光和红外瞬态吸收光谱技术来探测模型蓝藻色素系统TePixJ的光循环。动力学过程从皮秒到秒跨越多个数量级。胆汁结合袋中的局部变化发生在皮秒至纳秒之间，随后蛋白质结构发生更大规模的变化，包括第二硫醚键的形成和断裂在微秒至毫秒之间。

更新日期：2020-08-18

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